Location: Plant Gene Expression Center
Project Number: 2030-21430-008-00-D
Project Type: Appropriated
Start Date: Oct 1, 2010
End Date: Sep 30, 2015
1: To determine the ethylene biosynthetic capacity of the tomato plant. [NP 301, C 4, PS 4A] 2: To determine some of the molecular details of the regulation of the tomato genes by the hormone auxin. [NP 301, C 4, PS 4A]
Ethylene is a unique plant signaling molecule. The hydrocarbon gas, known as the fruit ripening hormone, controls many aspects of plant growth and development, and its synthesis is highly regulated. Recent experimental evidence indicates that the key enzyme in the ethylene biosynthetic pathway,1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) is encoded by a highly divergent multigene family in various mono and dicotyledonous plants. The members of each family are differentially regulated by various inducers and developmental stages. Studies on the heterodimerization properties of the various ACS isozymes are proposed in order to determine the biochemical capacity of the ACC gene family. Functional intermolecular complementation and biomolecular fluorescence complementation (BiFC) experiments will be carried to achieve this objective. Furthermore, molecular experiments are proposed to determine which of the Auxin Response Factor (ARF) gene family members regulates the various ACS genes in tomato.