Location: Genomics and Bioinformatics Research2011 Annual Report
1a. Objectives (from AD-416)
ARS and the International Center for Agricultural Research in the Dry Areas (ICARDA, Syria) will cooperate to provide support and oversight for cotton research conducted within Pakistan. ICARDA will also facilitate programs in Pakistan to address the need for improved crop management practices for small farmers, and coordinate workshops and training opportunities for Pakistani researchers with ARS designated researchers.
1b. Approach (from AD-416)
ICARDA, Syria will provide the support and oversight for projects conducted within Pakistan by Pakistani researchers, including, but not limited to screening for CLCuV resistance, establishing an in situ collection of cotton germplasm, transferring new sources of resistance into adapted varieties, and establishing sentinel plots to monitor the CLCuV spread and change in Pakistan. ICARDA will facilitate programs initiated to address the need for improved crop management practices for small farmers, and coordinate workshops and training opportunities for Pakistani researchers.
3. Progress Report
ICARDA is managing funds directed towards Pakistani institutes and has first line of oversight of productivity and expenditures of Pakistani institutes working on the U.S.A./Pakistani Cotton Enhancement Program that deals with Cotton Leaf Curl Virus (CLCuV) in Pakistan. Potential expenditures and productivity are then passed onto the ARS collaborator to assure their conformance to U.S.A. regulations. During the past year, a project manager was hired and working with the ARS collaborator a comprehensive plan with Pakistani partners is being developed. The present goal of the proposed three year program is to identify sources of resistance to CLCuV and transfer them into adapted lines. Cotton germplasm will be evaluated for CLCuV resistance; genetic mapping populations developed to identify DNA markers to assist in the transfer of the resistance to adapted breeding lines, and methods will be developed to monitor changes in CLCuV virulence and occurrence in Pakistan cotton production regions. As a secondary method of possible control, transgenic methods to combat the virus will be investigated. Listing of Pakistani partner institutes with a short responsibility descriptor is provided: Virus screening - National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad; Punjab University, Lahore. Location for holding and multiplying germplasm in Pakistan for seed sent from U.S.A. germplasm collection - Central Cotton Research Institute Sakrand, Sakrand. Testing of U.S.A. germplasm collection for CLCuV resistance - Cotton Research Station, Vehari; NIBGE; Central Cotton Research Institute, Multan. Small farmers agronomic practices to reduce CLCuV pressure - National Agricultural Research Center, Islamabad; Punjab Extension Service. Transgenic approaches for resistance - NIBGE; Centre of Excellence in Molecular Biology, Lahore. Research activities during this reporting period were monitored by the ADODR through email communications with the cooperator, phone conversations, Skype calls and through in-person discussions with the cooperator in Pakistan and Syria.