Location: Genomics and Bioinformatics Research2013 Annual Report
1a. Objectives (from AD-416):
Collaborate with ARS researchers to provide germplasm for Cotton Leaf Curl Virus (CLCuV) screening and transfer resistance identified into adapted cotton lines. Cooperative work with DREC will ensure timely production and transfer of seeds for Cotton Leaf Curl Virus (CLCuV) screening in Pakistan. Lines identified with resistance can be crossed to adapted cotton lines and the progeny evaluated quickly and efficiently. Resistant and susceptible lines of the same species can be crossed to develop mapping populations.
1b. Approach (from AD-416):
DREC will increase some of the germplasm accessions from the Cotton Germplasm Collection, and process seed for Cotton Leaf Curl Virus (CLCuV) screening. Putative resistant lines will be used as parents in crosses to transfer resistance to cotton lines adapted to U.S. cotton growing regions. Markers for resistance will be tracked in progeny of crosses involving parents resistant to CLCuV to confirm transfer of the resistance genes, and progeny will be evaluated under field and greenhouse conditions for suitable agronomic properties and fiber quality.
3. Progress Report:
The funding for this project originates from project 6402-21310-003-09R where additional details can also be found. Seeds from the U.S. Obsolete Variety Collection and a core set of Gossypium (G.) hirsutum from the national cotton collection, grown at Stoneville, MS, in 2011 were processed, packaged and sent to Pakistan for screening in summer of 2012. There were 113 lines from the Obsolete Variety Collection and 84 lines from the Core Set packaged and shipped to Pakistan. With help from the Mississippi State and local seed inspectors and the Pakistani officials, import permits and phyto-sanitary certificates were obtained for the shipment. Seed increases at Stoneville for 2012, were planted April 26th and with good growing conditions developed well and produced sufficient seed for screening in Pakistan in 2013. The 2013 field season, runs from February through November. The technician processed and packaged the seed for shipment to Pakistan. A new Research Technician was hired to assist with plot planting, data collection, plot maintenance and genotyping. January 16, 2013 a Research Technician came on board for the SCA technical support position.