1a. Objectives (from AD-416):
1) Produce a voucher collection of Bactrocera samples across subspecies from diverse range of populations through input from international collaborator and collecting trips. 2) Perform morphological identification of specimens and provide samples for generic analysis to develop species and population level markers. 3) Initiate species complex population studies, provide taxonomic ID of specimens, and curation of molecular data and insect specimens.
1b. Approach (from AD-416):
Using traditional methods of fruit fly taxonomy we will trap, curate, and identify fly specimens within the B. dorsalis species complex. This traditional work will be conducted by a post-doctoral researcher with prior training in B. dorsalis identification. Samples will be collected from the Pacific region, East Asia, and Australia and then transferred to UH Manoa for curation at the Department of Entomology Insect Museum. After morphological identification, these specimens will then be available to be genetically analyzed using currently developed phylogenetic markers and new markers identified from genome-wide analysis of reference specimens within USDA-PBARC and the University of Hawaii at Manoa labs. Formerly 5320-22430-023-07S; 6/2011.
3. Progress Report:
The goal of this research is to initiate species complex population studies, provide taxonomic ID of specimens, and curation of molecular data and insect specimens. This directly contributes to objective 5 of the in-house project, "Develop basic understanding of the oriental fruit fly genome, annotation of functional proteins that regulate development, metabolism, sensory reception and sex determination". We have continued to increase our collections of Bactrocera fruit fly specimens and continue to curate at the University of Hawaii Manoa Insect Museum. Sampling included trapping with methyl eugenol or cue-lure as well as collection from emergence from host fruit and includes samples from Thailand, Laos, Malaysia, Cambodia, Japan, China, Taiwan, French Polynesia, Hawaii, and west Africa representing over 50 individual populations. In the last year, we have focused on generating multi-gene sequence data for these specimens through collaboration between University of Hawaii, USDA, ARS,Pacific Basin Agricultural Research Center (PBARC), and Animal and Plant Health Inspection Service Center for Plant Health Science Technology (APHIS-CPHST). The placement of Bactrocera invadens within the B. dorsalis complex was completed, and it was demonstrated that invadens does not appear to be a unique species, but rather a member of the species complex. In addition, the complex appears to be paraphyletic in terms of species within the complex.