1a. Objectives (from AD-416):
1) To identify marker trait associations between elite North American oat varieties and total dietary fiber, beta glucan, disease resistance and agronomics using association genetics. 2) Develop predictive assays for total dietary fiber, beta glucan, and disease resistance. 3) Implement TDF and BG predictive assays in marker-assisted breeding to develop oat varieties with higher levels of each trait.
1b. Approach (from AD-416):
Six-hundred and twelve oat lines representing the diversity important to North American Oat breeding programs and elite lines from each breeding program will be grown in ID, ND, Saskatoon, and Winnipeg. Seed harvested from these locations will be milled in Aberdeen and assayed for total dietary fiber and beta glucan levels in Winnipeg. Disease data (Crown rust, Stem rust, and BYDV) will be collected in IN, IL, NY, WI, and MN. Genotypic and phenotypic data will be used to determine marker-trait association using JMP Genomics and TASSEL. An open array real-time TaqMan assay will develop to run predictive assay on marker-trait associations. The predictive assays will then be employed through the ARS Genotyping center in Raleigh, NC, to begin development of oat varieties with improved levels of TDF, BG, and disease resistance. Document Trust with Prairie Oat Growers Association. Log 40795.
3. Progress Report:
This is the final report for project 5366-21000-028-32T terminated in December 2012. This project relates to Objective 3 of the parent project, “Develop improved barley and oat cultivars meeting the needs of conventional and specialty markets for both dryland and irrigated production systems”. As part of the phenotyping for the Agronomy and Quality Testing of the Oat Single-nucelotide polymorphism (SNP) Development and Identification of Loci, 480 spring lines, 129 CORE lines and 170 winter lines were grown in Idaho oat breeding nurseries. In 2010, the 480 spring oat lines were planted in both Aberdeen and Tetonia; CORE and winter lines were grown in Aberdeen location only. Yield, test weight and plumpness were collected from Tetonia location. Data for yield, heading date, lodging, test weigh, plumpness, clean weight and gross weight were collected in Aberdeen location. In 2011, the spring and CORE collection materials were planted in both Aberdeen and Tetonia; the winter lines were planted in Aberdeen. Data for test weight, heading date, plant height, plumpness, and lodging were collected for all the materials. All the data were sent to cooperators in Canada for the data analysis. Data will be used to map quality traits in oat using SNP markers and an association mapping approach.