Project Number: 2040-22430-025-01-R
Project Type: Reimbursable Cooperative Agreement
Start Date: May 1, 2010
End Date: Apr 30, 2015
The objective of this research is to perform genome-wide analysis of several members of the Oriental fruit fly species complex with focus on: • Identification of gene regions for use in species-level diagnosis based on these genomes • Identification of gene regions that demonstrate poor performance for diagnostics (and should not be included in screening studies) • Identification of population-level markers for pathway analysis and population structure studies (these markers should be useful across species boundaries and provide markers for various bactrocera and fruit fly species) • Comparative phylogenomics analysis of the included species to direct future studies and test species boundaries
Comparative genomic analysis within the dorsalis complex will be performed by using pyrosequencing technology to generate several “shallow” (low coverage) genomes from Bactrocera species and analyze them using bioinformatic tools. Using five-ten fruit fly specimens representing Bactrocera species in the dorsalis complex our team will generate genomic databases for each specimen using a 454 pyrosequencer operated at the University of Hawaii. This genomic work will be designed and supervised by a PBARC research entomologist who is currently in charge of annotating the completed Bactrocera dorsalis genome. The species included for genomic analysis will be selected based on similarity to B. dorsalis, economic significance, and value to SIT programs. The data generated using 454 technology will be edited, annotated and analyzed by CPHST and ARS staff. Documents Reimbursable with USDA-APHIS-CPHST. Log 42092. Formerly 5320-22430-023-04R; 6/2011.