Location: Sunflower and Plant Biology Research
Project Number: 3060-21220-028-10-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2010
End Date: Jun 30, 2015
Development and evaulation of non herbicide-tolerant B. napus breeding populations using recently discovered sources of resistance and identify Brassica napus gerbicide-tolerant breeding lines with enhanced resistance to Sclerotinia sclerotiorum.
This proposal builds on results of previous projects that identified B. napus and B. rapa plant introduction materials and developed double haploid lines from Ames 26628, a B. napus accession with high levels of resistance to S. sclerotiorum. The project described here intends to develop and evaluate canola breeding populations using Ames 26628 DH lines and to continue efforts to identify herbicide-tolerant canola breeding lines with enhance resistance to S. sclerotiorum within the NDSU canola breeding program. To develop the breeding population, we intend to first produce DH materials from Topas, a public line which has been selected to be the susceptible parental line. F2 seeds from the Ames 26628 x Topas cross will be screened in the greenhouse using the petiole inoculation technique. Plant samples will be collected at this time for molecular marker evaluation. Resistant materials will be taken to seed production in the greenhouse and the following summer they will be evaluated in a disease nursery for their reaction to S. sclerotiorum as well as for agronomic traits. The identification of herbicidetolerant canola breeding lines with resistance to S. sclerotiorum will be conducted through replicated greenhouse and field trials. This project would contribute towards one of the goals of the Sclerotinia Initiative: the identification and characterization of new sources of resistance to manage diseases caused by this pathogen.