Location: Plant Science Research
Project Number: 6645-11000-008-10-S
Project Type: Specific Cooperative Agreement
Start Date: May 1, 2010
End Date: Mar 31, 2013
Map abiotic resistance genes in soybean for iron deficiency chlorosis, drought, salt stress, aluminum tolerance, and ozone tolerance.
Approximately 200 random inbred lines at the F5 stage are ready for seed increase during the summer of 2010 to generate the seed stocks that will be used as material for subsequent evaluations of environmental stress resistance. DNA extraction will be performed and SNP markers will be generated for each random inbred line of the population. A linkage map for the population will be constructed from the DNA data. The population will be evaluated for drought, iron deficiency chlorosis, toxic soil aluminum, salt, and atmospheric ozone pollution, the association of each trait with DNA markers (QTL analysis) will be performed. Independent preliminary research conducted by USDA-ARS in Raleigh, NC, and by the University of Minnesota identified a family of soybean genotypes from Fiskeby, Sweden, that are resistant or partially resistant to drought, iron deficiency chlorosis, toxic soil aluminum, salt, and atmospheric ozone pollution. A population developed from hybridization of Fiskeby III (representing the stress resistance of the Fiskeby soybean family) with Mandarin (Ottawa) (susceptible to drought, ozone, salt, and iron deficiency chlorosis, but resistant to aluminum) will be used to map resistance genes. The original hybridization was made in 2006 and seed of the resulting population were advanced by single seed descent.