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United States Department of Agriculture

Agricultural Research Service

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Location: Emerging Pests and Pathogens Research

2013 Annual Report

1a. Objectives (from AD-416):
The objectives of this cooperative research project are to develop and release improved potato cultivars with resistance to multiple races of the golden nematode, effect more efficient protocols to screen segregating potato populations for resistance to the golden nematode, develop profiles of new resistant cultivars to aid grower acceptance, provide for nuclear disease-free seed of resistant cultivars.

1b. Approach (from AD-416):
1) Crosses of potato clones will be made to generate populations that are segregating for resistance to multiple races of the golden nematode and improved horticultural and marketing characteristics. 2) Potato clones will be evaluated for nematode resistance. 3) Resistant potato clones will be subjected to an extensive evaluation scheme and selected for improved yield, desired marketing characteristics, and disease resistance. 4) Production and storage profiles for advanced clones will be developed through field testing. 5) Disease-free seed stocks will be maintained and multiplied as necessary to meet grower demands.

3. Progress Report:
Produced over 40,000 seeds of 22 progenies. We transplanted 12,000 seedlings (N generation) into 72 hole seedling trays and then to six inch pots in outdoor beds. After harvest, we saved 10,250 clones and they all segregate for resistance to GN Ro1. We also planted approximately 6,300 clones at the 4-hill plots stage (M generation) and saved 777 clones. We planted 1,123 white clones and 108 colored clones at the 20-hill plots stage (L generation). All clones segregate for resistance to Ro1 and/or Ro2. Three hundred and sixteen clones were saved after postharvest analyses. The 107 clones at the 100-hill plots stage (K generation) were also planted in 2 x 15 foot observation plots and 50 clones were saved. Thirty-five clones (J generation) were planted in replicated trials at one location and in a seed plot. Twelve clones survived post-harvest selection based on yield, appearance, and specific gravity. In addition, we tested 10 advanced round white and 4 round red clones (H, G, NY generations) at two locations around Ithaca and also distributed seed for several other trials. One of the white clones (NY140) is resistant to both Ro1 and Ro2. We continued to screen potato clones and varieties for GN resistance. A total of 441 and 59 clones were evaluated for Ro1 and Ro2 resistance, respectively. Among these, three hundred and twenty-five clones were identified to be resistant to Ro1 and thirty-nine clones were resistant to Ro2. Extension potato variety yield trials were conducted with growers in upstate New York. Marketable yield, tuber quality and appearance, maturity, storage life and processing potential are among the important characteristics that were evaluated. We continued to produce Foundation seed of advanced GN-resistant potato clones and varieties. In the 2012 growing season, 46 percent of the acreage at the Uihlein Farm of Cornell University was planted to 21 GN resistant varieties. The total estimated cwt (hundredweight) of GN resistant varieties sold and/or distributed was 4,835 cwt.

4. Accomplishments

Last Modified: 05/26/2017
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