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United States Department of Agriculture

Agricultural Research Service

Research Project: Alfalfa Germplasm Enhancement for Blue Aphid and Stem Nematode Resistance

Location: Plant Germplasm Introduction and Testing Research

Project Number: 2090-21000-031-02-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Apr 1, 2010
End Date: Mar 31, 2015

Screen dormant alfalfa germplasm for resistance to blue alfalfa aphid (Acyrthosiphon kondoi) and stem nematode (Ditylenchus dipsaci) and release germplasm.

Plant selected dormant germplasm in greenhouse for determination of resistance to blue aphid. At the same time, screen large numbers of plants from Columbia Basin College germplasm for selection purposes. Score resistance reaction according to standard tests using known non-dormant resistant varieties as resistant checks. If dormant resistant plants are identified, cross in greenhouse, test and reselect. If no resistance is identified in dormant germplasm, cross with resistant non-dormant germplasm in the greenhouse. Screen seed from dormant germplasm for resistance and plant in the field to select for class four dormant plants. Recombine in greenhouse for germplasm release. Standard tests developed for screening and testing for stem nematode have failed to produce resistant varieties that will perform well in the field. Recurrent field selection is a good indication that field selection may be better suited than standard screening procedures that have been in use for over 30 years with little or no progress. Columbia Basin College (CBC) will plant experimental varieties selected from infested fields for stem nematode to evaluate progress for resistance. Additional selections will be made from the field for seed increase and testing. Germplasm will be released based on test results. At the same time a greenhouse screening procedure will be developed that will allow more time for infection of all plants. More precision is necessary for progress toward higher levels of resistance.

Last Modified: 10/16/2017
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