Location: Crop Improvement and Protection Research
Project Number: 5305-22000-012-19-A
Project Type: Cooperative Agreement
Start Date: Jan 1, 2011
End Date: Jun 30, 2012
The specific objectives of this project are to 1) develop a rapid detection assay for Verticillium dahliae in spinach seed; 2) determine where the fungus is located in spinach seed to develop effective seed treatments; and 3) identify lettuce and spinach resistant to V. dahliae to limit spread via seed.
Detection of the fungus in vegetable and spinach seeds will be carried out by PCR techniques; localization of the fungus in spinach seed will be determined by confocal microscopy; and spinach and lettuce will be screened for resistance to Verticillium wilt in greenhouses.