Location: Wenatchee, Washington2010 Annual Report
1a. Objectives (from AD-416)
1. Phenotyping fruit maturation and ripening behaviors including fruit firmness, crispness, ripening season, fruit size, and storability for 6-8 cultivars and selected seedlings in Pink Lady x Honeycrisp progeny, in a developmental stage-specific fashion. 2. Characterizing expression patterns of 20-25 previously identified candidate genes, implicated in plant hormone biosynthesis, transport and response by qRT-PCR method and analyzing their association with the specific traits of apple fruit ripening pattern and quality attributes.
1b. Approach (from AD-416)
Apple fruit ripening behaviors and fruit quality attributes including ripening season, fruit firmness and fruit size will be characterized for selections in a "Pink Lady x Honeycrisp” progeny. Documents Trust with WA Tree Fruit Research Commission. Log 40944. The expression patterns of 20-25 previously identified candidate genes, implicated in plant hormone biosynthesis, transport and response will be analyzed by qRT-PCR method. Association of expression with the specific traits of apple fruit ripening pattern and quality attributes will be assessed.
3. Progress Report
This project is related to objective 2 of the associated in-house project which is to identify the genetic factors regulating fruit texture. Plant hormones regulate diverse plant physiological processes including apple fruit ripening and development of quality. The current knowledge regarding the “typical effects” for a given plant hormone are primarily from studies on non-fruit tissues such as root, shoot or leaf in model plant systems. This research project is built on our previous study using high density apple microarray and identifying key genetic factors influencing apple fruit ripening and fruit texture. The basic set-up is to test those identified candidate genes in a cross population, for the association between their expression patterns and fruit phenotypes; then the verified identities of those unigenes can be utilized to develop DNA sequences based molecular marker facilitating apple breeding program. Up to now, 8 candidate genes have been tested in this population, for their association with ripening season, fruit firmness and fruit size. Obtained data are under analysis. Progress is monitored through annual submission of written and oral progress reports to the funding agency.