Project Number: 5350-43000-006-11-T
Project Type: Trust Fund Cooperative Agreement
Start Date: May 1, 2010
End Date: Apr 30, 2013
1. Identify volatile compounds that accumulate during CA storage. 2. Characterize volatile compound dynamics during storage in atmospheres that induce low O2 and/or high CO2 injury. 3. Determine if recognition of changes in volatile compound production during low O2 or high CO2 stress has utility for CA system management. 4. Develop sampling protocols to enable detection of biomarkers for scald and other disorders.
An untargeted analysis of volatile compounds in controlled atmosphere chambers and rooms will be conducted using current analytical technologies. Impacts on volatile production from pre-storage treatments (DPA, SmartFresh), as well as timing and duration of O2 and/or CO2 stress will be evaluated. Analyses will primarily be conducted using gas chromatography/mass spectrometry which provides highly informative qualitative and quantitative results with high sensitivity.