1a. Objectives (from AD-416):
Objective 1: Develop a highly informative DArT-MAB genotyping platform complementary to the OOPA-MAB platform and benchmark both platforms. Objective 2: Conduct a two year multi-location association mapping study to develop marker-trait associations to facilitate the development of new oat varieties with superior health benefits and improved agronomics, milling quality, and disease resistance. Objective 3: Partner with the barley CAP portal to effectively utilize and disseminate the information generated by the project.
1b. Approach (from AD-416):
1. Genotyping: Two DArT discovery arrays from the PstI/TaqI genomic representation will be developed. The arrays will be used to genotype an oat reference panel, and at least one mapping population. From these, we will choose a robust and informative subset of 3,000 final DArT markers that will be arrayed on a second-generation DArT genotyping platform (DArT-MAB). 2. Field testing: A single row augmented plot design will be used to evaluate the oat association mapping population and checks in Winnipeg and Ottawa. Agronomic traits evaluated prior to harvest will include; 1) heading date, 2) plant height, 3) lodging, and 4) maturity. Each location will collect 500g of seed for shipment to Aberdeen, ID, where all processing and preparation for quality and nutritional component evaluation will occur. Quality traits measured in Aberdeen will include: 1) groat percentage, 2) percentage plump kernels, 3) 1000 kernel weight, 4) groat plumpness, 5) groat color, and 6) percentage broken groats. 3. Quality: 50 grams of ground meal from groats from two locations over two years will be sent to Winnipeg for determination of total dietary fiber and ß-glucan using standardized methods. 4. Data management: POOL will be expanded to contain the germplasm from this project, and will coordinate records so that they are mirrored in THT.
3. Progress Report:
Progress was made on the objective, which fall under National Program 301, Component 2, Crop Informatics, Genomics, and Genetic Analyses, Component 3: Genetic Improvement of Crops, and Component 4: Plant Biological and Molecular Processes. Progress on this project focuses on problem of assessment of genotyping using the new DNA markers, agronomic and quality traits of oat lines. Seed for lines submitted to the AFRI and CORE panels was increased and shipped to Aberdeen, Idaho, for inclusion in all North American trials. Phytosanitary certificates and export/import permits were obtained for all seed shipments as required. Field Trials at Lacombe, AB were grown as single yield plots again in 2011. Agronomic traits were evaluated during the growing season and plot yields were measured post-harvest. 500 grams of seed from each plot was carefully cleaned to prevent mixing of samples and shipped to Aberdeen, Idaho, in January 2012 for physical quality evaluation, after obtaining the appropriate phytosanitary documentation. All collected phenotypic data was sent electronically to the Project Director. Disease Nurseries were established in Winnipeg, Manitoba, in 2011. These were artificially inoculated with smut, crown and stem rust and adult plant reactions were evaluated for each of the 3 diseases. Seedling reactions to rusts were evaluated in greenhouses in Winnipeg. Nurseries grown at Glenlea, Manitopa, were artificially inoculated with barley yellow dwarf virus and reactions were recorded. Phenotypic data was annotated and shared electronically for inclusion in the CORE project database and for further analysis. In 2011, three Nurseries of AFRI-Spring, AFRI-CORE, and AFRI Winter lines were grown at Ottawa in yield plots. Agronomic and disease data were collected in the field, and yield and grain quality data were determined after harvest. Electronic data for 2011 were included in the CORE project database. Samples of 50 g per entry were prepared. Grain quality data for the 2011 nurseries are still to be gathered. Some entries in the AFRI-CORE panel were suspected to be incorrect, possibly due to seed mishandling at various stages. These issues have been largely resolved through the use of phenotypes and of molecular markers to diagnose and remove suspected is classifications. Total dietary fibre 1000 groat samples from 500 diverse genetic accessions grown at 2 locations were received and prepared for fibre analysis. Quality analysis is ongoing in a scientists AAFC grain chemistry laboratory in Winnipeg, Canada including beta-glucan (AACC Intl. Approved Method #32-23) and other dietary fibre components (AACC Intl. Approved Method #32-50.01). Results support Objective 3 in the parent project plan, development of improved oat lines. This is the final report for the project. During the life of the project, a total of 480 spring oat lines and 128 CORE lines including checks were evaluated for heading date, plant height, lodging, and maturity for two years. All the agronomic data have been collected and sent to the CORE database for mapping analysis. In addition, 50 grams of seeds of each line from each year was sent to Aberdeen, Idaho for milling quality traits analysis. After analysis, this data may enable plant breeders to more efficiently screen for milling quality in oat.