Location: Immunity and Disease Prevention Research2012 Annual Report
1a. Objectives (from AD-416):
To determine the effects of consuming fresh sweet cherries on markers for inflammation, immune status, cardiovascular disease and diabetes.
1b. Approach (from AD-416):
We previously conducted a study with Bing sweet cherries and published the results in the Journal of Nutrition in 2006. Because of a tight budget we could perform only a limited number of analyses at that time. Newer and more sensitive assays have become available since the time this study was conducted. We have plasma and white blood cell culture media samples obtained from our previous cherry study. We have recently procured the analysis of plasma samples from Rule Based Medicine for 89 antigens, by using the Human MAP Version 1.6. Initial analysis of these data shows that consumption of cherries altered the concentrations of several other markers. These results are very encouraging but need to be confirmed in our laboratory before we publish them. We will analyze the plasma samples for these select analytes by using the Meso Scale Diagnostic Technology in the Bioanalytical Support Laboratory at WHNRC. Our first priority is to complete the analysis of the plasma samples, if there are any funds left after that, we will analyze the cell culture media for select inflammatory markers. Results will be analyzed and findings published in a peer reviewed journal.
3. Progress Report:
This project supports objective 1 from the parent CRIS project. The overall focus of the parent CRIS is to investigate the role of dietary fatty acids and citrus limonoid glucosides (LG) on chronic inflammatory diseases including nonalcoholic fatty liver disease, insulin resistance, diabetes, and cardiovascular disease. Cherries are rich in polyphenols and have strong anti-oxidant and anti-inflammatory effects as LG. Thus, consumption of cherries can affect the risk for the diseases listed above. By using a targeted proteomic analysis we examined the biomarkers for these diseases in the plasma sample collected in a study we conducted in 2003. Thus, risk factors evaluated are the same, but the nutrient investigated was different. For this study we performed a targeted proteomic analysis for plasma samples from a previous human study in which study participants had consumed sweet Bing cherries for 28 days. Samples were analyzed for 89 proteins, (RBM, Human MAP Version 1.6) that include pro-and anti-inflammatory cytokines, growth factors, adhesion molecules, clotting factors, hormones, and markers for immune status, and cancer. We found significant reduction in several plasma markers of inflammation and cardiovascular disease. Within 28 days after discontinuation of cherry consumption, plasma concentrations of these markers completely or partially returned to the pre-supplement levels. These results are consistent with those from animal and cell culture models, and suggest a strong health promoting effect of sweet Bing cherries. The progress of the study has been frequently discussed by phone and e-mails with the sponsor of the study (WSFC) and collaborators, the director of human studies and director of Bioanalytical laboratory. The results were presented at the annual meeting of the WSFC in December 2011.