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United States Department of Agriculture

Agricultural Research Service


Location: Small Grains and Potato Germplasm Research

2010 Annual Report

1a. Objectives (from AD-416)
Numerous studies, using a wide variety of species, have been conducted to reduce/eliminate fish meal from aquaculture feeds. However, variability in sources and inadequate chemical characterization of dietary ingredients has been cited as primary limitations when comparing results from these various studies. Recently, the Plant Products in Aquafeeds Working Group (PPA) developed a Strategic Plan to advance the development of fish meal free diets through improved research coordination. A primary objective of goal number one of that strategic plan is the standardization of feed input compounds used in research studies. Standardization of feed ingredients is complicated by the fact that the small ingredient quantities needed by most research groups limits their ability to obtain consistent commercial products. Additionally, it is generally cost prohibitive for individual researchers to fully characterize both the nutrient and anti-nutrient profiles of each ingredient used in experimental feeds. For these reasons, a pilot program is proposed to address these needs and provide this service for the research community.

1b. Approach (from AD-416)
Through this new program, small quantities (~25 kg/ ingredient/shipment) of characterized ingredients will be made available to interested researchers. Each ingredient will be supplied with an analysis of nutrients (proximate composition, and amino acids) and anti-nutrients (specific anti-nutrients dependant on ingredient). These analyses will include but are not limited to; ): trypsin inhibitor activity (ISO 14902) (AOCS, 1983); stachyose, raffinose, and oligosaccarides concentrations were determined using HPLC and a calcium column; group A saponins were separated and quantified using methods described by Shiraiwa et al ( 1991a); group B saponins were separated and quantified using non-starch polysaccrides including ADF, NDF crude fiber. All ingredients will be characterized using consistent protocols. Commercial sources of soybean meal and soy protein concentrate will be used and will be donated by the supplier or alternatively one of the collaborators. The only obligations of receiving the various ingredients will be the acknowledgement of the sponsors as the ingredient source and providing all sponsors with a reprint of any manuscripts published using these ingredients. A four layered approach to developing widespread participation in the program will be used. First, a 20 minute presentation detailing the project will be given at during the Alternative Feeds Special Symposium at Aquaculture America in San Diego, March 2010. Second, the program will be described on three websites including, the USDA Trout-Grains Project, and USFWS Bozeman Fish Technology Center. Third a project description will be sent to all members of the Plant Protein in Aquafeeds Working Group which includes many international contacts. Lastly, a poster describing the project will be presented at the 14th International Symposium on Fish Nutrition and Feeding in Quindao, China, May 2010. Documents Reimbursable with United Soybean Board. Log 41094.

3. Progress Report
Pilot Program to Characterize Feed Ingredients for the Development of Plant based Aquaculture Feeds. This project has been initiated and feed ingredients , soy bean meal, soy protein concentrate, modified soy meal (fungal modification), and corn protein concentrate, have been obtained and sent out for analysis. Ingredients were provided to two research institutions and more will be sent upon receipt of the analyses. Communication and coordination was achieved through frequent emails and phone conversations.

4. Accomplishments

Last Modified: 06/21/2017
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