1a. Objectives (from AD-416):
1) To identify marker trait association between elite North American oat varieties and key traits including total dietary fiber, beta glucan, vitamin E, crown and stem rust resistance, barley yellow dwarf resistance, height, lodging, plumps, test weight. 2) Develop and use the marker-trait association for predictive assays through the USDA, ARS Genotyping centers by specifically placing the equipment needed to perform the assays.
1b. Approach (from AD-416):
Six-hundred and twelve oat lines representing the diversity important to North American Oat breeding programs and elite lines from each breeding program will be grown in ID, NY, IL, IN, WI, MN, ND, TX, LA, NC, Ottawa, Saskatoon, Winnipeg, and the UK. Agronomic traits will be collected by the breeder in at least ID, NY, Saskatoon, and Winnipeg. Seed harvested from these locations will be test for milling quality for avenanthramide, a-tocotrienol, a-tocopherol, total dietary fiber, and ß-glucan. Disease data (Crown rust, Stem rust, and BYDV) will be collected in TX, IN, IL, LA, ID, WI, Ottawa, and MN, while freeze tolerance data will be collected in NC. Genotypic and phenotypic data will be used to determine marker-trait association using JMP Genomics and TASSEL. An open array real-time TaqMan assay will develop to run predictive assay on marker-trait associations. Documents Trust with N. American Millers Association. Log 39993.
3. Progress Report:
Progress in this project helped further Objective 2 in the parent project aimed at developing methods to accelerate breeding for adaptive traits in oat. All proposed experiments were completed. Major accomplishments from the research include: 1) A genetic marker resource was developed for oat. A total of 6,000 SNP markers were identified and used to develop high-throughput genotyping assays based on the Illumina microarray chip platform. These assays were then used to characterize a selection of elite oat lines from North American research programs. These data are now being used to build a consensus oat map that will improve the existing current map (see next point) 2) The first genetic map of oat was developed that had the correct number of linkage groups. 3) All the phenotypic and genotypic data collected have been compiled and distributed to all members of the North American Collaborative Oat Research Enterprise as a resource for further research.