1a. Objectives (from AD-416)
1) To identify marker trait association between elite North American oat varieties and key traits including total dietary fiber, beta glucan, vitamin E, crown and stem rust resistance, barley yellow dwarf resistance, height, lodging, plumps, test weight. 2) Develop and use the marker-trait association for predictive assays through the USDA, ARS Genotyping centers by specifically placing the equipment needed to perform the assays.
1b. Approach (from AD-416)
Six-hundred and twelve oat lines representing the diversity important to North American Oat breeding programs and elite lines from each breeding program will be grown in ID, NY, IL, IN, WI, MN, ND, TX, LA, NC, Ottawa, Saskatoon, Winnipeg, and the UK. Agronomic traits will be collected by the breeder in at least ID, NY, Saskatoon, and Winnipeg. Seed harvested from these locations will be test for milling quality for avenanthramide, a-tocotrienol, a-tocopherol, total dietary fiber, and ß-glucan. Disease data (Crown rust, Stem rust, and BYDV) will be collected in TX, IN, IL, LA, ID, WI, Ottawa, and MN, while freeze tolerance data will be collected in NC. Genotypic and phenotypic data will be used to determine marker-trait association using JMP Genomics and TASSEL. An open array real-time TaqMan assay will develop to run predictive assay on marker-trait associations. Documents Trust with N. American Millers Association. Log 39993.
3. Progress Report
This is a joint project with the oat SNP development and identification of loci affecting key traits, project number 5366-21000-028-028-11R. Therefore the progress report is the same to date with the addition of a new project website. A population of 685 oat lines was selected by ten North American breeding programs containing lines expressing valuable traits like high soluble fiber and resistance to particular diseases. Seed for each line was purified in Aberdeen, ID and increase over the winter in New Zealand. Seed for the population was shipped to over 15 locations across the globe and agronomic traits like heading date and lodging were evaluated. In addition, disease reactions to barley yellow dwarf virus, crown rust, and stem rust were recorded for the population in select locations. Based on sequence information from the General Mills Trust project number 5366-21000-028-07T, 700 new oat genetic markers were developed. Additionally, a new database called The Avena Toolbox (TAT) has been established. Communication and exchange of work has taken place via meetings, and conference calls