1a. Objectives (from AD-416)
The objectives of this cooperative research are to: 1) genotypically and phenotypically characterize plant pathogens that cause diseases of crops grown in cereal-based cropping systems, 2) to genotypically and phenotypically characterize microbial biocontrol agents that suppress plant pathogens in these cropping systems, and 3) to determine the molecular genetic and biochemical basis of plant-microbe interactions and plant defense.
1b. Approach (from AD-416)
Plant pathogens and their biocontrol agents will be characterized through a combination of molecular fingerprinting techniques and methods of classical plant pathology and microbiology. The population structures of pathogens and biocontrol agents will be determined by statistical analysis of DNA banding patterns generated by techniques such as rep-PCR and RAPDs, RFLPs, and AFLPs, and by sequence analysis of 16S rDNA and key genes. Results of genotypic and phenotypic analyses will be compared. Plant-microbe interactions will be characterized using a combination of laboratory, greenhouse and field studies, and molecular biology and bioanalytical tools. Biologically active metabolites produced by pathogens and antagonists will be characterized using techniques such as high performance liquid chromatography (HPLC) and mass spectrometry. Documents SCA with WSU.
3. Progress Report
This SCA was recently initiated thus there is no progress to report. Accomplishments from this project will align with Components 1, Problem Statements 1A and 1B; Component 2, Problem Statements 2C; Component 3, Problem Statement 3B, and Component 4, Problem Statements 4A, 4B and 4C of NP 303. Progress on this project is monitored by weekly conversations with the collaborator.