1a. Objectives (from AD-416)
The objective of this cooperative research project is to identify genes important in resistance of soybean to the soybean cyst nematode and to develop soybean plants with broad resistance to soybean cyst nematode.
1b. Approach (from AD-416)
Soybean genes expressed during nematode invasion will be identified that may be used to broaden resistance to SCN through soybean transformation or targeted mutations. Virtually nothing is known about the resistance or susceptible response of soybean to SCN. These genes can be over- or under-expressed in soybean roots and challenged with SCN. Genes will be identified that are up regulated during the resistance response using microarrays or genes localized to known SCN resistance quantitative trait loci (QTLs). These up-regulated genes will be over-expressed using strong constitutive promoters or under-expressed using RNA interference. Transgenic roots with altered expression profiles for candidate resistance-associated genes will be challenged with different races of SCN and the resistance profile examined.
3. Progress Report
The objective of this agreement is the development of molecular technologies leading to soybean with greater resistance to the soybean cyst nematode. Thirteen genes were sequenced and cloned in the area around a major locus (Rhg1) conferring soybean cyst nematode resistance. This is funded by the United Soybean to work on developing soybean with resistance to the soybean cyst nematode. Progress is monitored via the submission of quarterly progress reports provided by Ohio State and one annual meeting is held in February to monitor progress. Conference calls and e-mails were also used to monitor and coordinate progress.