1a. Objectives (from AD-416)
1. To collect the ex situ and/or in situ volatile emission of select native and non-native oak trees (Quercus spp.). 2. To analyze collected volatile organic compounds (VOCs) via GC-MS and identify possible chemotaxonomy trends and/or chemotype identifiers of selected trees to various insect pests. 3. To identify VOCs with kairomonal behavior to select insect pests.
1b. Approach (from AD-416)
To accomplish the stated objectives the following approach will be utilized by the graduate student under the mentorship of a Professor and ARS scientist. 1. Perform ex situ VOC analysis on a large portion of selected oak tree (Quercus spp.) leaves to determine general VOC emission trends. 2. Utilize the in situ small-scale collection method (collection bag, SPME) of the USDA-ARS PMR unit to acquire VOCs from select trees identified from ex situ analysis. 3. Separate and identify collected VOCs via GC-MS analyses. 4. Develop a chemotaxonomic tree based upon trends of identified VOCs and herbivore damage. 5. Utilize electrophysiological and/or behavioral bioassays to determine kairomonal activity. Documents NFCA with UC Davis.
3. Progress Report
All volatile collections have been performed, the volatiles separated and identified via gas chromatography mass spectrometry GC-MS, and the data are currently being analyzed.