1a. Objectives (from AD-416):
Objective 1: Characterize genetic and phenotypic contributions of important production traits for Morone broodstock management and improvement. Sub-Objective 1A. Produce experimental hybrid striped bass families. Sub-Objective 1B. Assess the genetic basis of phenotypic variation of growth in hybrid striped bass. Sub-Objective 1C. Evaluate the performance of hybrid striped bass families under alternate stocking rates. Objective 2: Refine nutrient requirements, evaluate alternate sources of protein, and develop practical feed formulas for Morone culture. Sub-Objective 2A. Refine essential amino acid requirements of advanced juvenile hybrid striped bass using practical ingredients. Sub-Objective 2B. Improve the performance of commercial hybrid striped bass diets in which fish meal is replaced with by-products of poultry processing or a blend of plant products. Sub-Objective 2C. Develop practical feed formulas for hybrid striped bass culture. Objective 3: Develop strategies to improve production system efficiency. Sub-Objective 3A. Define stocking rate/biomass-yield relationship in a mixed suspended growth production system. Sub-Objective 3B. Compare catfish yields in a mixed suspended growth production system scaled up to ponds.
1b. Approach (from AD-416):
Identify and characterize genetic variation in commercially relevant traits of white and striped bass and implement a breeding program to develop superior hybrid striped bass parental breeding stocks. Evaluate growth performance of half-sibling families of hybrid striped bass reared communally in earthen ponds. Evaluate the performance of hybrid striped bass families under alternate stocking rates. Define requirements for hybrid striped bass for first-limiting indispensable amino acids in all plant protein diets. Evaluate amino acid supplementation for hybrid striped bass diets in which fish meal is replaced by alternative feed ingredients. Develop and evaluate in aquaria and tank culture, and validate in earthen pond culture diets for hybrid striped bass formulated with alternative feed ingredients. Evaluate the effects of stocking density, feeds and feeding strategies, and environmental conditions on survival and growth of hybrid striped bass and catfish in tanks and earthen ponds. Evaluate novel, intensive production technology in tanks and earthen ponds.
3. Progress Report:
Broodstock were conditioned and spawned, resulting in 48 half-sibling sunshine bass crosses that yielded 1,080,000 larvae and 19 half-sibling white bass crosses that yielded 94,000 larvae. Broodstock for the next phase of spawning are currently being conditioned. A study was also initiated to assess phenotypic variation in growth of 40 half-sibling families of sunshine bass in earthen ponds managed to maintain minimum dissolved oxygen concentrations greater than 25, 40, or 55% of the complete saturation concentration. In addition, a companion study was initiated in tanks to evaluate performance of industry pool hybrid striped bass exposed to constant dissolved oxygen concentrations of 25, 40, or 100% of saturation. Targeted genetic markers allowed the scientists to determine how fish cope with stressful low oxygen conditions at the molecular level. A study was completed to investigate how adult striped bass, white bass, and hybrid striped bass were able to cope with short-term exposure to low dissolved oxygen concentrations. A study was completed using recirculating aquaculture systems to evaluate the effect of three feeding regimes, and with or without the benefit of additional algae, on larval hybrid striped bass growth, survival, and early development of selected digestive enzymes. Standard growth and condition measurements and tissue sample analyses are ongoing. In collaboration with scientists from Montana State University and the USFWS Bozeman Fish Feed Technology Center, MT, dose-response trials evaluating the effect of dietary inclusion of grain distillers dried yeast on growth of carnivorous fish were completed. In collaboration with scientists from Southern Illinois University, Carbondale, and the USFWS Bozeman Fish Feed Technology Center, MT, studies were initiated to evaluate nutrient composition and nutrient availability from two sources of Asian carp meal for use in hybrid striped bass, catfish, cobia, and rainbow trout diets to assess their fishmeal replacement value. In collaboration with a researcher at the University of Arkansas at Pine Bluff, we conducted an experiment using a factorial arrangement to determine the effects of partial replacement of dietary fish oil with linseed oil, and the addition of a probiotic to the diet, to determine the effects on growth, body composition, immune response, and resistance to heat shock in hybrid striped bass. In collaboration with a researcher from the University of Arkansas at Pine Bluff, work was initiated on a grant funded by the Arkansas Soybean Promotion Board titled, Minimizing Use of Fishmeal in Hybrid Striped Bass Diets Using Non-GMO Soybeans Selectively Bred for Use in Aquafeeds. A study was completed quantifying the effect of fingerling stocking rate on the production of stocker catfish, and resulting development of phytoplankton communities and associated common off-flavors in a biofloc technology production system. An ensuing study was initiated to determine the effect of three stocking rates with large channel catfish stockers on production, water quality dynamics, and the development of common off-flavors in a biofloc technology production system.
Beck, B.H., Fuller, S.A., Peatman, E., McEntire, M.E., Darwish, A.M., Freeman, D.W. 2012. Chronic exogenous kisspeptin administration accelerates gonadal development in basses of the genus Morone. Comparative Biochemistry and Physiology. 162A(3):265-273.