1a. Objectives (from AD-416):
Objective 1: Characterize genetic and phenotypic contributions of important production traits for Morone broodstock management and improvement. Sub-Objective 1A. Produce experimental hybrid striped bass families. Sub-Objective 1B. Assess the genetic basis of phenotypic variation of growth in hybrid striped bass. Sub-Objective 1C. Evaluate the performance of hybrid striped bass families under alternate stocking rates. Objective 2: Refine nutrient requirements, evaluate alternate sources of protein, and develop practical feed formulas for Morone culture. Sub-Objective 2A. Refine essential amino acid requirements of advanced juvenile hybrid striped bass using practical ingredients. Sub-Objective 2B. Improve the performance of commercial hybrid striped bass diets in which fish meal is replaced with by-products of poultry processing or a blend of plant products. Sub-Objective 2C. Develop practical feed formulas for hybrid striped bass culture. Objective 3: Develop strategies to improve production system efficiency. Sub-Objective 3A. Define stocking rate/biomass-yield relationship in a mixed suspended growth production system. Sub-Objective 3B. Compare catfish yields in a mixed suspended growth production system scaled up to ponds.
1b. Approach (from AD-416):
Identify and characterize genetic variation in commercially relevant traits of white and striped bass and implement a breeding program to develop superior hybrid striped bass parental breeding stocks. Evaluate growth performance of half-sibling families of hybrid striped bass reared communally in earthen ponds. Evaluate the performance of hybrid striped bass families under alternate stocking rates. Define requirements for hybrid striped bass for first-limiting indispensable amino acids in all plant protein diets. Evaluate amino acid supplementation for hybrid striped bass diets in which fish meal is replaced by alternative feed ingredients. Develop and evaluate in aquaria and tank culture, and validate in earthen pond culture diets for hybrid striped bass formulated with alternative feed ingredients. Evaluate the effects of stocking density, feeds and feeding strategies, and environmental conditions on survival and growth of hybrid striped bass and catfish in tanks and earthen ponds. Evaluate novel, intensive production technology in tanks and earthen ponds.
3. Progress Report:
In collaboration with a feed company, we initiated an experiment to evaluate diet digestible protein and amino acid supplementation on the performance of hybrid striped bass (HSB) in summertime pond production. In collaboration with scientists from Southern Illinois University, Carbondale, and the USFWS Bozeman Fish Feed Technology Center, MT, studies were completed to evaluate nutrient composition and availability, and fishmeal replacement value of 2 sources of Asian carp meal for diets for HSB, catfish, cobia, and rainbow trout. In collaboration with the University of Arkansas at Pine Bluff, tissue analyses and statistical modeling were completed in a study to determine the effect of partial replacement of dietary fish oil by linseed oil and addition of probiotic to the diet on HSB growth, body composition, immune response, and resistance to heat shock. In collaboration with researchers from the UA at Fayetteville and at Pine Bluff, we continued work on the Arkansas Soybean Promotion Board-funded project, Minimizing Use of Fishmeal in Hybrid Striped Bass Diets Using Non-GMO Soybeans Selectively Bred for Use in Aquafeeds. We completed analysis on samples evaluating the effect of different salinities on nutrient availability from a fishery by-product meal, yeast protein, barley protein concentrate, canola protein concentrate, corn protein concentrate, menhaden fish meal, and Spirulina algae. In collaboration with a scientist from the Plant Genetics Research Unit, Columbia, MO, we initiated a study with HSB to evaluate dietary inclusion of soybeans that lack anti-nutritional factors. We completed a study to determine the histidine requirement for juvenile HSB and initiated a second trial to establish the whole organism histidine metabolism. We completed a larval HSB growth and survival study comparing dispersed kaolin clay, green water (algae present), and clear water culture. We completed a study to determine the effect of 3 stocking rates of large channel catfish stockers on production, water quality dynamics, and the development of common off-flavors in a biofloc technology production system. We initiated a study to assess phenotypic variation in growth of 28 half-sib families of HSB in earthen ponds at the approximate upper and lower limits of Phase II stocking rates used by industry. We initiated a study to quantify the effect of 2 solids removal rates on water quality and catfish production in a biofloc technology production system. We conditioned broodstock and produced 61 half-sib HSB crosses that yielded 1,900,000 larvae. We optimized total RNA isolation methods for HSB muscle and liver tissue, extracted RNA from HSB reared at summer pond temperatures, fed diets of varying ingredient and nutrient profiles and are analyzing RNA expression profiles. In collaboration with an Auburn University researcher, we initiated work on the USDA National Research Support Project-8-funded project, Moronid Reference Transcriptomes. In collaboration with researchers at Universities of New Hampshire, Maryland, and Connecticut, we initiated work on the Northeastern Regional Aquaculture Center-funded project, Striped Bass Selection for Marine Culture.
Beck, B.H., Fuller, S.A. 2012. The impact of mitochondrial and thermal stress on the bioenergetics and reserve respiratory capacity of fish cell lines. Journal of Aquatic Animal Health. 24(4):244-250.