1a. Objectives (from AD-416)
Develop multiple disease resistant soybeans through gene discovery and transfer of disease resistance genes into soybean.
1b. Approach (from AD-416)
The overall approach will include the following three steps: 1) determine functionality of candidate genes involved in resistance to sudden death syndrome, Sclerotinia stem rot, brown stem rot, Rhizoctoinia root rot, and charcoal rot diseases using this bean pod mottle virus-based VIGS system, 2) confirm the resistance reaction in soybean using RNAi gene silencing in soybean, and 3) recover transgenic lines of soybean with resistance to multiple diseases of soybean.
3. Progress Report
Using the bean pod mottle virus-virus induced gene silencing (BPMV-VIGS) system, plants with candidate silenced or over-expressed defense-related genes were evaluated for disease resistance. We initially found genes encoding Glycine max SCaM4, Annexin, lipase, acid phosphatase and WRKY40, and showed four other genes: GmLRK3, Lox9, chlorophyllase2, and phytoene desaturase to be promising as well for altering the response of soybean when inoculated with the rust pathogen. We have also done similar work in inoculating plants for Phytophthora root rot. This inoculation technique is being modified to accommodate the later inoculation of plants needed in working with the BPMV-VIGS system. The other pathogens that we scheduled to test need further modification of the inoculation methods to accommodate the BPMV-VIGS system. This project was monitored through electronic messages, phone conferences, and face-to-face meetings.