Location: Cereal Disease Lab2013 Annual Report
1a. Objectives (from AD-416):
Identify sources of seedling and adult plant resistance to wheat stem and leaf rust, and oat crown rust; Map resistance genes and QTLs for resistance to cereal rusts; and Use molecular and virulence markers to determine pathogen population structure and migration patterns.
1b. Approach (from AD-416):
Screen oat and wheat seedlings for rust resistance in the greenhouse; Screen oat and wheat adult plants for resistance in the field to rusts and FHB; Screen recombinant inbred (RI) populations for rust resistance in the greenhouse and field; and Use DArT and SSR markers to genotype RI populations; Determine virulence phenotype and molecular (SSR) genotype of rust and FHB pathogen isolates from the U.S.
3. Progress Report:
We characterized fungal communities associated with the soil-plant interface of four different plant species grown in monoculture and in the presence of other plant species. We developed reference databases for and sequenced DNA libraries of fungi from these samples. We utilized clustering and alignment-based methods to test for shifts in fungal community structure and diversity in response to plant community dynamics and soil characteristics.