1a. Objectives (from AD-416):
To functionally characterize soybean response to pathogens, with focus on Sclerotinia sclerotiorum. Candidate defense-related genes will be identified from soybean response to the pathogen or products of the pathogen. Functional characterization will include repression and/or enhancement of expression of the candidate genes in soybean and/or Arabidopsis.
1b. Approach (from AD-416):
The ARS scientist will identify candidate defense genes, whereas the collaborator's lab will assist with construct design for over and under expression of genes in host plants and will generate transgenic soybean material. The ARS scientist will assist in propagation of the soybean transgenics and will conduct disease assays on this material to determine effect on defense.
3. Progress Report:
The collaborating lab has generated lines of transgenic soybean to use to study candidate defense genes. Homozygous lines are being developed to be used in disease assays. The collaborating lab has conducted standard PCR and quantitative RT-PCR to verify that the plants do contain the proper RNAi constructs, and that the constructs lead to inhibition of the RNA corresponding to the target genes.