Location: Emerging Pests and Pathogens Research2010 Annual Report
1a. Objectives (from AD-416)
The overall objective of this proposal is to refine the current management strategies based on industry needs to reduce overall levels of PVY and prevent the continued spread of necrotic and recombinant strains.
1b. Approach (from AD-416)
Our overall objective in this proposal is to refine the current management strategies used by the certification agencies to reduce the initial virus inoculum planted in the field and those used by growers to reduce the spread of virus within the crop. Specifically, we will 1. Work with seed certification agencies to monitor PVY strains and develop new diagnostic and training tools to better identify and eliminate PVY from seed stocks. "Specialty Crops Research Initiative" 2. Work with breeding programs to better recognize and screen for virus resistance and symptom expression. 3. Work with growers to develop cost effective virus control strategies for both minituber and conventional seed production that can be implemented on the farm.
3. Progress Report
This report documents the progress of an SCRI – NIFA grant to an ARS PI and eight other co-PIs located at ARS, Aberdeen, ID, and the Universities of Idaho and Wisconsin. Information here is specific to ARS progress; information on progress specific to the University of Idaho or Wisconsin can be found in the reports 22000-018-23G or 22000-018-21G, respectively, or in the reports submitted to SCRI-NIFA. This is the first year of the grant and there are no documented impacts from the research as of yet. In FY2010 we focused on 3 objectives; 1) Which of the North American cultivars are susceptible to potato tuber necrotic ringspot disease (PTNRD). Greenhouse trials have been completed on 7 cultivars and 2 advanced breeding lines. Each entry is challenged with multiple strains of PVY. Foliar symptoms are recorded on a weekly to bi-weekly basis and tuber symptoms are recorded at harvest and after storage. Two cultivars and one breeding line were susceptible to PTNRD. Field trials are scheduled for 2011 and 19 putative PVY resistant lines have been identified from 9 breeding programs across the U.S. Virus-free minitubers are currently in production to provide the seed for 2011 for replicated trials at 3 sites in Idaho, Wisconsin, and New York. 2) Continued surveillance and assessment of the PVY strains affecting the major seed production regions of the U.S. In January, samples were collected from the post harvest tests from NY, ME, MI, WI, NE, ND. Fifty symptomatic samples were collected from WI and ME, fewer from the other states due to flooding and freeze damage. Most samples were photographed and frozen. Analyses include Multiplex RT-PCR on frozen potato tissue, inoculation to tobacco, ELISA on tobacco tissue using 4C3 and 1F5. Most of the isolates recovered are the ordinary strain of PVY, but the tuber necrotic strains are present in samples from most states. 3) Produce training manuals that will assist seed certification inspectors and growers to identify the various foliar and tuber symptoms induced by the various strains of PVY predominant in that region. This year photographs of infected leaves are being collected from the post harvest test plots and from greenhouse experiments on PTNRD symptoms. Isolates infecting the tissue and cultivar identities are included with each picture. A website has been developed that will serve as the primary communication tool for the grant participants and the primary tool for outreach to the potato and greater scientific communities. The website will contain updated information on the objectives and research progress of the grant as well as educational, outreach and extension materials for all sectors of the potato industry. The website also has a discussion forum feature that will allow registered users to post questions, comments and answers regarding PVY and other viruses affecting potato. The website is expected to be live by August 2010. Progress is monitored via conference calls every other month, emails, and a face-to-face meeting in March.