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United States Department of Agriculture

Agricultural Research Service

Research Project: Development of Molecular Markers to Identify Different Physiological Races of Pathogens Responsible for Soilborne Diseases of Alfalfa

Location: Plant Germplasm Introduction and Testing Research

Project Number: 2090-21000-031-01-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 22, 2009
End Date: Sep 21, 2014

Identify molecular markers specific to Aphanomyces eiteiches Race 1 and 2.

Collect fungal isolates from various geographical regions. Extract DNA from each and combine from each respective race (bulked DNA). Use RAPD, SRAP, or other primers to screen for polymorphisms specific to each race. Develop SCAR markers from sequence derived from the latter markers. Documents SCA with WSU.

Last Modified: 10/17/2017
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