Project Number: 6435-43440-044-03-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Aug 1, 2009
End Date: Jun 30, 2013
Use empirical tools to improve computational prediction models for detecting clinically relevant linear IgE binding sites.
a. Synthesize known and computationally predicted cross-reactive IgE binding epitopes of peanuts and tree nuts on a membrane. b. Use clinically well-characterized patient sera with known peanut and/or tree-nut (walnut and almond or cashew) allergy to assess the clinical relevance of IgE binding to a particular synthetic peptide. c. Continuously optimize computational model based on empirical data obtained from goal b.