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United States Department of Agriculture

Agricultural Research Service


Location: Plant Stress and Germplasm Development Research

Project Number: 3096-21000-019-05-N
Project Type: Non-Funded Cooperative Agreement

Start Date: Dec 9, 2009
End Date: Jun 14, 2014

The objectives of this research are to evaluate and validate improvements within ARS in-house breeding lines in yield and fiber quality traits through comparative field evaluations, and to evaluate and identify sources of Fusarium wilt (FOV) resistance in a diverse cotton germplasm collection, including commercial and experimental cultivars and mapping populations, through field and greenhouse trials.

Acala, non-Acala Upland, Pima, and other Gossypium cultivars and germplasm from a broad genetic background will be evaluated for yield and fiber quality properties at both Shafter and West Side Research and Extension Centers of the University of California, and in commercial fields when available. In addition, for Fusarium wilt resistance (FOV), cotton entries will be evaluated at the University of California Kearney Research and Extension Center greenhouses, and in verified FOV race 4 or race 1 infested commercial fields when available. Completely randomized or randomized complete block designs with three or four replications will be used for most trials. A minimum of two replications will be used for yield or FOV resistance estimation when seed availability or study acreage is limited. Plot size in field studies will vary depending on objectives and seed and land availability. In studies focused on yield characters, data collected will include in- and end-of-season measurements of plant height, number and type of nodes, and boll position, retention, and numbers per plant. Fifty-boll and six-pound samples of seed cotton will be collected to estimate lint percentage and fiber quality properties. These cotton samples will be ginned at Shafter to determine lint percent. Where fiber quality is of key interest samples will be sent for analysis to one or more commercial classing laboratories or to the USDA Classing office in Visalia, CA. Studies focused on identification of FOV resistance will assess a diverse cotton germplasm collection, which includes diploid cottons, public germplasm, and germplasm developed by conventional breeding techniques. Measured responses specific to FOV studies will include stand survival rates, ratings of disease severity, and vascular staining. Results of FOV field trials will be supplemented by replicated studies under greenhouse conditions, which will document responses of cultivars or breeding lines to artificially-inoculated soil mixes. Several races of FOV (e.g., races 1 and 4) may be used in greenhouse evaluations to better understand variations in exhibited germplasm resistance responses. In addition, we will evaluate selected control practices such as seed treatment, planting date, and irrigation regimen under different soil types for management of the FOV disease in combination with resistant or tolerant cultivars. Documents NFCA with University of CA-Davis. Formerly 5303-21220-003-16N (11/10).

Last Modified: 10/17/2017
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