Location: Avian Disease and Oncology Research2011 Annual Report
1a. Objectives (from AD-416)
As described in grant entitled “Development and field evaluation of genome-wide marker-assisted selection (GWMAS) over multiple generations in commercial poultry,” the consortium that includes members from Wageningen University will: 1. Refine the Theoretical and Molecular Aspects of GWMAS; and 2. Field Assessment of GWMAS
1b. Approach (from AD-416)
For Objective #1, alternative methods of utilizing GWMAS will also be explored. Principal Component Regression (PCR) and Partial Least Squares (PLS) are two methods that were mainly developed in chemometrics to deal with situations where the number of effects to be estimated greatly exceeds the number of records. The estimation of 10-100,000 haplotype effects from ~1,000 phenotypic records is exactly such a situation, and thus may suit these methods very well. Semi-parametric regression was suggested by Gianola et al. (2006) for the estimation of GW-EBVs. Its main advantages are: (1) it ‘automatically’ deals with non-additivity of gene effects, whereas the other methods, in their basic form, assume additive gene action; and (2) it makes few distributional assumptions about the data, which could make this method more robust in practical situations. A computer simulation study based on data generated by Muir’s (2007) program will be conducted to compare the statistical methods Ridge Regression (RR), BLUP, GRM, BayesB, Xu’s (2003), PCR, PLS, and semi-parametric regression for their computational efficiency and accuracy of predicting breeding values in poultry breeding programs, their bias (over/under prediction) of true breeding values, their sensitivity to the assumptions that are made about the genetic model, and the number of records and genotypes that are computationally feasible. The simulated genome structure will largely follow that of Meuwissen et al. (2001), but the parameters determining the genome structure, such as effective population size, mutation rates and distribution of mutational effects, gene action (additivity, dominance, epistatic effects), admixture of populations, etc., will be varied, in order to assess the sensitivity of the methods to differences in the structure of the genome. For Objective #2, the expected breeding values (EBV) will be computed for each normalized trait of each animal based on genotypic information and phenotypes (if present). These EBV would in turn be used to determine total merit by use of the appropriate weights given by the companies. The companies will do their own BLUP evaluations based on phenotype as per their usual breeding programs. The goal is to make selection decisions based on the EBVs for each trait and/or total merit the same for each method of selection [BLUP, GWMAS], the only difference being the way in which the EBVs will be estimated.
3. Progress Report
This project is directly linked to projects 3635-31320-008-37R, Specific Cooperative Agreements 3635-31320-008-20S, 25S and 31S, titled “Development and Field Evaluation of Genome-Wide Marker-Assisted Selection (GWMAS) Over Multiple Generations in Commercial Poultry." To meet the growing demands of consumers, the poultry industry will need to continue to improve methods of selection in breeding programs for production and associated traits. One possible solution is genome-wide marker-assisted selection (GWMAS). First proposed by one of our team members, GWMAS utilizes markers spanning the entire genome to increase accuracy and efficiency of estimating breeding values (EBV). This new method promises significant benefits, but there are many unanswered questions calling for proof that GWMAS actually works. Retrospective analysis has shown genome-wide marker-based EBV correlate highly with phenotypic Best Linear Unbiased Prediction (BLUP) EBV. However, there are concerns that these analyses will not reflect reality once implemented because selection may rapidly change variances, allele frequencies, and generate unfavorable linkage disequilibrium (LD) which only becomes apparent after the second round of selection. As planned, two meat-type and three egg-type chicken pure lines are being selected in parallel using either traditional or GWMAS. This year, after completing two rounds of selection, we conclude that compared to birds selected in parallel using current state-of-the-art breeding methods, genomic selection is superior for the vast majority of the traits selected including body weight and breast yield. This research strongly suggests that genomic selection is an improved breeding method. If costs for genetic testing continue to go down, then poultry breeders should be able to economically breed chickens faster using genomic selection and adapt more readily to changing consumer demands. The economic impact could be great since with 1 million meat-type birds processed per hour in the US alone, the net effect of even small improvements are large and worth millions of dollars. This project is monitored by weekly e-mail and telephone calls between the parties, a monthly teleconference and, when possible, direct interactions at scientific meetings.