Project Number: 5012-22000-021-03-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: May 21, 2009
End Date: May 20, 2014
Soybean yields are affected quantitatively and qualitatively by fungi, insects, nematodes, and viruses. The ability of pathogens and pests to colonize and/or infect soybean plants is the result of interactions of pathogen and host genes that allow pathogenic organisms to reproduce and cause disease. The objective of this cooperative research project is to identify and characterize the expression of soybean and pathogen genes involved in the establishment and/or maintenance of disease-causing interactions.
Candidate soybean genes that support/permit pathogen accumulation or that are involved in pathogenesis will be identified through the genetic analysis of soybean lines differing in their susceptibility to pathogens and pests. In addition, genes expressed at the host-pathogen interface will be identified and characterized using genetic and expression profiling techniques. The involvement of the genes in disease will be confirmed by gene-specific complementation and/or gene silencing. Pathogen genes will be identified by similar genetic and expression profiling techniques. Host and pathogen proteins that physically interact in diseased cells will be identified through in vivo interaction studies and in vitro protein binding and affinity chromatography analyses.