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ARS Home » Midwest Area » Ames, Iowa » National Laboratory for Agriculture and The Environment » Agroecosystems Management Research » Research » Research Project #414874

Research Project: Stripping Antibiotic Resistance with STRIPS: Evaluating Prairie Buffer Strips to Mitigate Resistance Genes from Manure Amended Fields

Location: Agroecosystems Management Research

Project Number: 5030-13000-011-63-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Feb 15, 2018
End Date: Feb 14, 2022

Objective:
1. Determine the effectiveness of prairie strips to retain Antimicrobial Resistance (AMR) from manure-amended croplands. 2. Characterize AMR mitigation in an existing prairie strip. 3. Characterize AMR fate and transport in a model prairie strip system to evaluate and predict performance and optimize design for AMR mitigation.

Approach:
Objective 1 uses simulated rainfall and runoff in small plots located at the interface of cropland with prairie strips to examine strips mitigation. The use of simulated rainfall and runoff allows experimental control of runoff and manure application. Objective 2 expands the work proposed in Objective 1 to the full landscape scale, allowing for further evaluation of AMR mitigation by prairie strips and investigating the mechanism of contaminant removal under natural conditions (rainfalll and runoff). This objective allows us to intensively test our hypothesis that the export of manure-derived antibiotics, antibiotic-resistant bacteria (ARBs), and antibiotic-resistant genes (ARGs) to surrounding soils and waters is reduced in prairie strip-treated agriculture when compared to manure-treated cropland. In Objective 3, we will construct a model of prairie strips mitigation in a state-of-the-art experimental flume system allowing us to evaluate and optimize the performance of prairie strips under controlled flow conditions. Samples of runoff water, manure, and soil will be analyzed using DNA-based techniques to assess the abundance of a variety of antibiotic resistance genes and mobile genetic elements.