1a. Objectives (from AD-416)
Freezing injury is a major source of risk and yield loss in winter wheat (Triticum aestivum L. em Thell) production. Our long-term goal is to improve frost tolerance in cultivated wheat.
1b. Approach (from AD-416)
We will use known gene sequences to identify and sequence genes on wheat chromosomes. Primers will be developed and used to resequence. Populations will be developed and we will determine the effect of each on frost tolerance. Documents SCA with UC Davis.
3. Progress Report
The question is whether mutations in the CBF12, 14 and 15 genes in wheat affect cold tolerance in mutant populations of tetraploid and hexaploid wheat. Our methods have been to focus initially on the A genome genes to develop genome specific primers to identify them and to identify mutant lines in a TILLING population created in ‘Kronos’ durum wheat. Genome specific primers were developed for CBFA12, CBFB12, CBFA14, and CBFB14 as well as a non-specific primer pair amplifying CBFA15 and CBGB15. A total of 16, 12, and 11 mutations have been discovered in these genes in the Tilling population and the CBFA-12 mutants have been sequenced. These results will be used to study the gene expression and cold tolerance of the mutant plants in order to predict how to manipulate these genes in order to develop wheat with better winter survival. Monitoring of this project was done via email communication (monthly) meetings with the PI (In Sept 2009 and January 2010) and a site visit in Sept, 2009.