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United States Department of Agriculture

Agricultural Research Service

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Research Project: Breeding Pierce’s Disease Resistant Table and Raisin Grapes and the Development of Markers from Seus Sources of Resistance

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-010-14-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Jul 1, 2008
End Date: Jun 30, 2013

Develop Pierce’s disease (PD) resistant table and raisin grape germplasm/cultivars with fruit quality equivalent to standards of present day cultivars. Develop molecular markers for Xf/PD resistance in a family from southeastern United States (SEUS).

1) Backcross BC1, BC2, BC3 hybrids (F8909-08 V. arizonica source of resistance) that have been selected for Pierce’s Disease (PD) resistance to high quality V. vinifera seedless table and raisin grapes. 2) Backcross F1 and BC1 resistant bunch grapes from native SEUS source of PD resistance as identified by greenhouse tests with high quality V. vinifera seedless table and raisin grapes. 3) Use embryo rescue to facilitate the hybridization of seedless parents. 4) Use molecular markers to select resistant F8909-08 backcross seedlings. 5) Select seedlings with highest fruit quality and test in the greenhouse to determine resistance. 6) Field test best resistant selections for PD resistance under natural conditions. 7) Perform production and quality trials of selections that have commercial potential. 8) Develop rough framework map of V. vinifera table grape X BD5-117 family to locate PD resistant QTL and develop molecular markers for marker assisted selection. Documents Trust with the Consolidated Central Valley Table Grape Pest and Disease Control District. Log 35507.

Last Modified: 08/18/2017
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