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United States Department of Agriculture

Agricultural Research Service

Research Project: ACS3 genotype of apple (Malus X Domestica) and its effect on 1-MCP treatment efficacy

Location: Wenatchee, Washington

Project Number: 5350-43000-006-06-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Aug 15, 2008
End Date: Aug 14, 2013

Characterize the expression patterns of ACS3 genes among elite apple cultivars, and elucidate the relationship of ACS3 expression and effectiveness of MCP treatment as well as subsequent postharvest fruit quality.

1. Fruit tissues representing different developmental stages will be collected for 12-16 cultivars, starting 30 days after full bloom till commercial maturity. 2. Apple fruits harvested at commercial maturity will be treated with1-MCP and fruit firmness changes of stored fruit will be assessed for at least 3 months. 3. Total RNAs will be isolated from above-mentioned fruit tissues using established procedure. 4. Gene-specific primers will be utilized to examine the expression patterns and dynamics on major ethylene biosynthesis enzyme encoding genes by quantitative real-time PCR methodology.

Last Modified: 08/21/2017
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