Location: Virus and Prion Research2011 Annual Report
1a. Objectives (from AD-416)
The objective of this cooperative research project is to characterize field isolates of swine influenza A virus (SIV) for the purpose of utilizing this knowledge to develop improved prevention and control programs.
1b. Approach (from AD-416)
Swine flu is caused by SIV, and is one of the top 3 disease concerns for US pork producers. Influenza A viruses can change or mutate through 2 general mechanisms. The first one is known as antigenic drift, this a process by which the virus mutates at a slow rate that is dependent on how frequently there is a mistake in replicating the virus genome. This mistake results in random changes in the nucleic acid sequence. The second process is antigenic shift. The genome of influenza A viruses is composed of 8 segments. When 2 different flu viruses infect the same cell it is possible for one or more of these segments to be switched between the different viruses. This switching of gene segments between viruses is call antigenic shift and this process can account for rapid genetic changes. In addition to drift and shift, swine are susceptible to infection with avian and human influenza A viruses. This phenomenon also contributes to rapid genetic change for SIV. The above described mechanisms for genetic change when coupled with modern swine husbandry make swine flu a dynamic disease. The potential for rapid genetic/antigenic changes has made this disease difficult to control through the use of commercial vaccines. In response, there is a growing trend to utilize autogenous vaccines, but they are not the complete answer. The NADC swine influenza project has focused on improving vaccines by developing vectored and attenuated SIV vaccines; and by in vivo and in vitro characterization of SIV field isolates. This research program requires a team of scientific and technical staff. Due to the recent departure of skilled staff, it is necessary to develop a working relationship with others that have a molecular skill set working with negative-sense RNA viruses. Thus the proposal for scientists from the NADC and ISU-CVM to collaborate on in vivo and in vitro characterization of SIV field isolates. The influenza A virus research will focus on studies that will 1) characterize viruses at a genetic level, 2) development a reverse genetics system, and 3) use this reverse genetics system to study virus function.
3. Progress Report
The focus of the project has been studying a recently discovered influenza A viral protein, PB1-F2. The exact function of this protein is not fully understood, nor why some viruses express the protein and others viruses do not. Investigating the function of PB1-F2 requires the production or purchasing of a number of reagents that should be specific for interaction with PB1-F2, but can be identified in virus-infected cells under certain conditions. One such reagent appears to cross-react with another cellular protein that we believe is not an influenza virus protein. A no-funds extension of the project to December 31, 2011 was implemented to more completely determine the possible relevance of this reagent-protein cross reactivity. Methods used for monitoring include phone contact, email, and meetings.