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United States Department of Agriculture

Agricultural Research Service

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Location: Genetic Improvement for Fruits & Vegetables Laboratory

2010 Annual Report

1a. Objectives (from AD-416)
The objectives of this project focus on determining the underlying genetic bases of quality attributes and nutritive value of Solanaceous vegetables in order to facilitate their deployment in improved germplasm. Diverse Solanaceous germplasm resources exist for enhancement of cultivated forms of tomato, pepper and eggplant. Genes that may be valuable for crop improvement are often not well characterized or they may be associated with undesirable traits. Over the next 5 years we will focus on the following objectives: 1) Develop tomato germplasm with enhanced fresh- and processing-market quality. Research will focus on identification of QTL and candidate genes that contribute to fruit quality, principally fruit firmness attributes. Breeding lines and genetic stocks will be released by ARS for development of germplasm with improved firmness attributes. 2) Develop new Capsicum germplasm with improved culinary and/or ornamental quality. Genetic mechanisms underlying tissue-specific anthocyanin accumulation and fruit flavor will be characterized. Germplasm with unique tissue specificity for anthocyanin/carotenoid pigmentation in foliage, flowers and fruit will be introgressed into genotypes with novel plant habits and fruit shape, size, configuration and flavor. The inheritance of morphological characters will be determined. New novel germplasm will be released. 3) Determine the inheritance of eggplant antioxidant content, principally phenolic acids that influence postharvest quality, in populations developed from crosses of diverse accessions in the eggplant core subset.

1b. Approach (from AD-416)
(Objective 1) QTL and candidate genes that contribute to fruit quality, principally fruit firmness attributes, will be identified in an inbred backcross line population developed from parental lines originating from interspecific Solanum lycopersicum x S. galapagense crosses. These lines exhibit divergent combining ability for fruit compression, puncture resistance and fruit fresh weight. A growing database of mapped SNP markers is available and will be utilized to identify and map firmness QTL. (Objective 2; Sub-objective 2.A) Utilizing divergent genotypes and environmental treatments, we have previously demonstrated differential expression of Capsicum anthocyanin-related structural and regulatory genes in tissues that vary in anthocyanin pigmentation. A dual approach using environmental and genetic mediated modification of anthocyanin pigmentation, combined with TEV-induced silencing will provide multiple avenues to characterize regulation of tissue-specific pigmentation in Capsicum. The effect of light treatment and silencing induced by TEV infection on anthocyanin structural and regulatory gene expression will be characterized. The siRNA/miRNA populations of anthocyanin pigmented and non-pigmented tissue will be compared to evaluate the contribution of small RNAs to regulatory gene expression. Gene expression and small RNA populations will be similarly assessed in a small series of recombinant inbred lines that we have developed which vary in tissue specific anthocyanin pigmentation. (Sub-objective 2.B) Germplasm with unique specificity for anthocyanin/carotenoid pigmentation in foliage, flowers, and fruit will be introgressed into genotypes with novel plant habits and fruit shape, size, configuration and flavor. Novel flavor attributes identified in exotic Capsicum germplasm will be characterized and introgressed into C. annuum. Backcross, pedigree and inbred backcross breeding will be utilized. Inheritance of morphological traits will be determined. Analyses will be performed using segregating F2, F3 and backcross populations that we have developed. QTL for flavor attributes will be identified using the principles described in Objective 1 for tomato fruit firmness. (Objective 3) Eggplant accessions with divergent fruit phenolic acid constituents and total phenolic acid content have been selected to determine the inheritance of individual classes of phenolic acid compounds and total phenolic acid content. Segregating F2 backcross populations to respective high and low phenolic acid parents will be developed and utilized to determine the inheritance of total phenolic acid content and individual classes of phenolic acids. Gene action, genetic factors and heritability estimates will be derived.

3. Progress Report
Pepper Genetics. Within the genus Capsicum there is abundant genetic diversity for plant habit and fruit and leaf characteristics for creating new plant types to meet today’s consumer needs. Building upon our previous award winning and patented cultivars, new breeding lines with novel character attributes and plant ideotypes were advanced to commercial trials for evaluation in culinary and ornamental applications. This germplasm afford growers with innovative opportunities to produce a high value alternative crop. A new CRADA is being developed to support this research. Related research utilizes exotic germplasm (see Accomplishments) for pepper fruit quality improvement. We identified Capsicum baccatum accessions with superior fruit quality attributes. Selected lines were genotyped and have been utilized to develop mapping populations and introgression lines for identification of genes associated with fruit flavor and related quality attributes and introgression into cultivated germplasm. The research addresses customer interest in improved product quality. Anthocyanin Genetics. Research is ongoing to investigate the regulation of anthocyanin biosynthesis in Solanaceous species. New results we have obtained from eggplant (see Accomplishments) suggests that additional regulatory elements influence pigment accumulation and that regulation differs among Solanaceous species. We have also isolated miRNA and sequenced miRNA from pepper to identify regulatory elements that interact with anthocyanin structural and regulatory gene products. Analysis of the data set is underway. The results are relevant to the evolution of anthocyanin color genetics and enhanced pigmentation in Solanaceous crops. Phytonutrients. An abundance of phytochemicals with putative human health benefits have been described. Additional information on their inheritance and efficacy is needed to develop new plants with superior nutritive value. Eggplant fruit phenolic acids contribute to fruit nutritive value and culinary quality. Building upon our previous research on phenolic acids, we have reconfirmed phenolic acid profiles from earlier analyses in a mapping population developed by cooperators to identify genes important for product quality. The results will facilitate development of superior eggplant germplasm.

4. Accomplishments
1. Pepper Fruit Quality Genetics. Capsicum baccatum is an exotic pepper species recognized as a potentially valuable source of genes for pepper quality improvement. However, this germplasm is poorly characterized and genes that may be valuable for crop improvement have not been identified. Utilizing selected C. baccatum accessions, we identified over 100 fruit volatiles and considerable variation in the concentrations of capsaicinoids and capsaicinoid analogs which contribute to fruit flavor and nutritional quality. The results identified valuable genetic diversity for fruit quality constituents and are important for continued progress in improvement of value-added pepper cultivars with improved flavor and nutritive value. This research is cooperative with the ARS Food Quality Laboratory in Beltsville and a CRADA partner. Manuscripts describing current and prior research have been held by the CRADA partner to protect intellectual property.

2. Anthocyanin gene regulation. Diversity exists in eggplant for fruit color which ranges from white to violet to black. The genes responsible for the biosynthesis of the anthocyanin compounds which impart color are not well characterized. A major gap in our knowledge exists concerning regulatory mechanisms which control accumulation of anthocyanins. We evaluated expression of regulatory and structural genes in genetic stocks of eggplant with contrasting anthocyan pigmentation in fruits and flowers and determined that anthocyanin structural and regulatory gene expression was required for pigment accumulation. The results demonstrated that different genetic mechanisms account for eggplant pigmentation in comparison to other Solanaceous species. A manuscript describing the results is in preparation. The results are important for elucidating the genetic control of pigment biosynthesis and development of new plants with enhanced fruit quality and nutritive value.

Last Modified: 07/25/2017
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