Location: Immunity and Disease Prevention Research2009 Annual Report
1a. Objectives (from AD-416)
Our objectives are to determine: 1) the relative absorption (plasma response) of beta-cryptoxanthin (CX) and beta-carotene (BC); 2) the relative transfer of CX and BC to breastmilk; and 3) the relative Vitamin A (VA)potential of CX and BC (breastmilk retinol responses).
1b. Approach (from AD-416)
Exclusively breastfeeding Bangladeshi women with marginal vitamin A status will be randomly assigned to receive 0.5 mg RAE/d, 6 d/week, for 6 weeks, as either 1) tangerines (CX), 2) orange sweet potatoes (BC), or 3) 0.5 mg RAE as retinyl acetate plus a low carotenoid fruit (apple). Breastmilk and plasma samples will be collected at baseline and weekly for measurement of CX, BC and VA. Test foods will be provided as daily snacks between meals, twice per day. The change in plasma and breastmilk concentrations of CX, BC and VA will be calculated and examined by treatment group using analysis of covariance, and used to compare the effectiveness of CX- and BC-rich fruits and vegetables as sources of vitamin A to the mother and child. Documents Reimbursable NRI Grant. Log 35592.
3. Progress Report
Our objective is to compare the effectiveness of two food based interventions meant to increase vitamin A status in lactating women with low vitamin A status: orange-fleshed sweet potatoes (an excellent source of beta-carotene) and mandarin oranges (a good source of beta-cryptoxanthin). To do this, we are conducting a human intervention study in Bangladeshi women. Human study protocols were finalized, translated, and approved by the University of California Davis and the International Centre for Diarrhoeal Disease Research,Bangladesh. Supplies and equipment were purchased and tested on site in Bangladesh. Carotenoid concentrations in orange-fleshed sweet potatoes and mandarin oranges were measured and used to determine the amounts of sweet potatoes and mandarin oranges that will be supplied to the study participants. We have completed two rounds of recruiting for the study. The UC Davis portion of the project is being monitored by monthly visits and frequent e-mails and phone calls. The project in Bangladesh is being monitored by frequent e-mail exchanges and infrequent phone calls. Also, the UC Davis collaborator has made one three-week site visit to Bangladesh, and the Bangladeshi collaborator made one two week visit to both WHNRC and UC Davis to exchange information and ideas.