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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Research Project #413321


Location: Corn Insects and Crop Genetics Research

2008 Annual Report

1a. Objectives (from AD-416)
Objective 1: Identify and utilize fertility/sterility mutants in a phenotypic recurrent selection program for insect-mediated cross-pollination to increase hybrid seed production. Objective 2: Identify phenotypes/genotypes that can be characterized and molecularly mapped that contribute to insect-pollinator attraction and reward.

1b. Approach (from AD-416)
Soybean accessions in the USDA germplasm collection will be crossed with known cytoplasmic restorer lines to identify cytoplasmic male sterile cytoplasms. Sterility mutants (nuclear) obtained via gene tagging from the w4-mutable system will be characterized genetically. Both the cytoplasmic and nuclear sterility mutants will be used in a phenotypic recurrent selection system, with insect-mediated cross-pollination, to increase hybrid seed production. The plant factors that contribute to the increased attraction/reward of the insect pollinators will be identified, characterized, and molecularly mapped.

3. Progress Report
The search for cytoplasmic male sterility in the USDA soybean germplasm was initiated and manual cross-pollinations were made. Newly identified male-sterile mutants were cross-pollinated to test for allelism with mutants in the Genetic Type Collection. Plant-progeny rows were evaluated and selection will be made for Population 2 to molecularly map the insect-pollinator attraction and reward traits. Plant samples for nectary starch have been analyzed and data processed for publication. This project 3625-21000-054-00D, Identification of Plant Genetic Mechanisms that Influence Insect-Pollinator Preference fits within NP301 Plant Genetic Resources, Genomics, and Genetics Improvement, Component 3 because germplasm with increased pollinator attraction are being developed.

4. Accomplishments

5. Significant Activities that Support Special Target Populations