Location: Hard Winter Wheat Genetics Research2010 Annual Report
1a. Objectives (from AD-416)
The objectives of this cooperative research project are: using genomics tools to 1) identify new QTL for FHB resistance in Asian landraces and U.S. hard winter wheat germplasm from U.S. and 2) transfer and pyramid QTL for FHB resistance from diverse sources to locally adapted cultivars using marker-assisted selection.
1b. Approach (from AD-416)
Use of high throughput molecular marker technologies to identify and isolate desirable genes/QTL from wheat germplasm materials collected from Asia and the southern Great Plains of the USA and to transfer and pyramid these QTL into adapted U.S. hard winter wheat cultivars using marker-assisted backcross to develop hard winter wheat cultivar/germplasm with improved resistance.
3. Progress Report
Fusarium head blight (FHB) is one of the most important constraints on wheat production and quality in the U.S.A. Use of FHB resistant cultivars is the most effective approach to control the disease and lower deoxynivalenol (DON) mycotoxin accumulation in wheat grain. The objectives of this cooperative research project are using genomics tools to: 1) identify new genes for FHB resistance in Asian landraces and U.S. hard winter wheat germplasm lines, and 2) transfer and pyramid genes for FHB resistance from diverse sources into locally adapted cultivars using marker-assisted selection. Two recombinant inbred line (RIL) populations from the crosses between two Chinese landraces, Haiyanzhong and Huangfangzhu, and Wheaton (a highly susceptible parent) were evaluated for FHB resistance. The data were analyzed and quantitative trait locus (QTL) analysis showed that Haiyanzhong has a QTL on wheat chromosome 7D and explained about 22-30% of variation for percentage of scabbed spikes (PSS). In Huangfangzhu, two QTLs with major effects on PSS were mapped on chromosome 3BS (near resistance gene Fhb1) and on chromosome 7AL. The manuscripts for the mapping work are in preparation. A RIL mapping population (cultivar Heyne x Trego) was evaluated for FHB in both field and greenhouse for FHB resistance. Heyne is a local HWW cultivar with moderate resistance to FHB. Parents were screened with 1000 DNA markers to identify polymorphisms. Work will continue identifying chromosome regions that affect disease severity. A new QTL was mapped on chromosome 7A near the centromere using a population of 200 chromosome recombinant inbred lines. This QTL showed a larger effect on PSS and explained 25% of the phenotypic variation. Progress on this agreement is monitored by regularly discussing program goals, approaches, and results (on site) and by reviewing annual accomplishments reports.