Location: Subtropical Horticulture Research2012 Annual Report
1a. Objectives (from AD-416):
1. Conserving tropical/subtropical ornamental crop genetic resourses and associated information important for the evaluation and utilization of that material. 2. Development and application of new or improved horticultural evaluation procedures and genetic marker-based approaches for genetic diversity assessment of the preceding genetic resourses. 3. Genetic improvement efforts directed to developing new, superior tropical/ subtropical ornamental germplasm. 4. Transfer of technology in the form of tropical/subtropical ormamental crop genetic resourses, associated information, and new, superior tropical/subtropical ornamental germplasm to researchers and breeders worldwide. 5. Evaluate containerized subtropical/tropical ornamental germplasm for its growth responses to different container media components. Where feasible, estimate for this germplasm the heritabilities and other patterns of genetic variation for adaptation/tolerance to the different container media components.
1b. Approach (from AD-416):
Collect new accessions of subtropical/tropical ornamental plants that have commerical potential in the U.S. Evaluate newly collected accessions and existing material, primarily flowering trees and shrubs already on site, using phenotypic characters and molecular makers. Organize the information such that it can be used as descriptors for inclusion in the GRIN system. Utilize the molecular and phenotypical analysis to breed improved germplasm in support of the ornamental industry. Communicate and coordinate subtropical/tropical ornamental horticultural research with local and regional industry, universites, and foreign germplasm effort in Florida, Puerto Rico, and Hawaii.
3. Progress Report:
Germplasm of 12 Bauhinia species have been planted in the field; these were grown from seed requested of the Desert Legume Collection. A particularly floriferous and well-adapted clone of Handroanthus impetiginosus (formely Tabebuia avellandeae) has been identified in the seedling evaluation block that we established. All population of Zamia from Jamaica and Dominican Republic have been genotyped 30 microsatellite loci. Zamia sample collections were completed for the Cayman islands. We have 10 single copy genes that are being used on 10 individual samples of each population genotypes with the simple sequence repeats (SSRs), and the sequencing has been completed for Jamaica, Puerto Rico and Dominican Republic. Analysis of WRKY gene sequences for all other genera of palm tribe Cocoseae (tribes Elaeidinae and Bactridinae) are complete, and a paper is being prepared for publication. analyzed. Patent applications are being prepared for five Hippeastrum hybrid clones and will be submitted to the Technology Transfer patent committee for consideration. We are increasing micropropagated stocks of two diploid Hippeastrum clones to increase attempt chromosome doubling with oryzalin early in 2012. Completed study on alternative potting substrate. Results show composted insect colony waste (CW) substitutions of between 30 and 70% for equal amounts of compost plus peat resulted in plant performance greater than or equal to that observed with a commercial mix. Composted CW is a nutrient rich material that can be used in 50% CW:50% peat mix will have good characteristics for a basic potting substrate for most container grown plants. One peer reviewed publication a second in in-house review). Completed study on ornamental species that have the potential for use in remediation of low-level arsenic contaminated areas. Currently data is being analyzed for manuscript preparation. Data collection on the effect of nitrogen fertilization on coralloid root development in cycads has been completed and these data are being analyzed.
Meerow, A.W., Gideon, M., Kuhn, D.N., Mopper, S., Nakamura, K. 2011. The genetic mosiac of iris series Hexagonae in Florida: inferences on the Holocene history of the Louisiana irises and anthropogenic effects on their distribution. International Journal of Plant Science. 172(8):1026-1052.