Location: Vegetable Crops Research2011 Annual Report
1a. Objectives (from AD-416)
Objective 1: Determine the genetic basis of and initiate selection for carrot, onion, cucumber, and melon quality attributes influencing human nutrition and health, disease resistances, and yield and quality components, and stress tolerance in cucurbits, and perform field performance and quality trials. Objective 2: Utilize current biotechnology to discover and evaluate genetic variation and to map agriculturally important traits in Allium, Cucurbit, and Daucus germplasm, and to develop genetic and breeding stocks. Sub-objective 2.A. Construct genetic maps of nuclear and organellar genomes using candidate genes, SCARs, SSRs, SNPs, transposon insertions, BACs, and cytogenetic stocks. Sub-objective 2.B. Fine map pigment and carbohydrate genes in carrot and onion, resistance genes for nematode in carrot and viruses in cucurbits, and epistasis, yield and quality components in cucumber. Sub-objective 2.C. Perform marker-assisted selection of carrot nematode resistance, onion male sterility, and cucurbit yield and quality. Sub-objective 2.D. Evaluate transgene escape in cucurbits. Sub-objective 2.E. Determine transposon mobility in carrot.
1b. Approach (from AD-416)
The long-term potential for improving a crop is only as great as the breadth of diversity that breeders utilize. Objective 1 targets evaluation and genetic characterization of carrot, onion, cucumber, and melon germplasm for traits important to growers and consumers. Discovery Goal 1 - Identify unique phenotypic variation in germplasm collections and breeding stocks to improve nutritional and processing quality, disease resistance, stress tolerance, and yield of Allium, Cucurbit, and Daucus vegetables, genetically characterize observed variation and initiate genetic incorporation of these phenotypes into elite germplasms. Many biotechnological tools have been developed to improve the efficiency of crop improvement. Objective 2 evaluates and develops these tools of carrot, onion, cucumber, and melon improvement. Identify adequate DNA polymorphisms in elite onion, cucumber, melon, and carrot germplasm to construct genetic maps for marker-facilitated selection of major horticultural traits. Discovery Goal 2.A – Identify adequate DNA polymorphisms in elite onion, cucumber, melon, and carrot germplasm to construct genetic maps for marker-facilitated selection of major horticultural traits. Discovery Goal 2.B – Evaluate variation at candidate genes in pigment and carbohydrate biochemical pathways for mapping in onion, cucurbit, and carrot. Discovery Goal 2.C – Identify and utilize markers to accurately identify desirable genotypes for male sterility restoration in onion, cucurbit yield, and carrot nematode resistance. Discovery Goal 2.D – Appraise the potential benefit(s) that transgenes might confer on transgenic populations using the ELISA test to estimate the degree of viral infection in wild populations and to determine the potential risk of virus gene introgression from commercial transgenic cultivation. Discovery Goal 2.E - Determine if native transposable elements in the carrot genome, such as DcMaster, and introduced ones, such as maize elements Ac and Ds transpose to new chromosomal regions.
3. Progress Report
USDA experimental carrot breeding entries were grown in Holtville, CA. Yield components evaluated root length, shape, forking, smoothness, tip shape, length, uniformity, premature bolting, vigor, earliness & powdery mildew. Quality components include color, smoothness, flavor, texture & nutritional value. Resistance to nematodes & Alternaria leaf blight was evaluated in CA & WI, respectively. New USDA hybrids performed very well in the trial as did USDA germplasm. Flavor evaluation was also performed for all entries. For carrot candidate gene evaluation, four flavonoid pathway genes & two additional nematode resistance genes were identified & genetically mapped. Families were developed to determine the genetic basis of carrot cytoplasmic male sterility restoration, alternaria resistance, & additional nematode resistance. Carrot transposable element variation was evaluated but not observed in a wild relative of carrot from North Africa. A large collection of onion germplasms were screened for resistance to pink root & Fusarium. Evaluated crosses among different sources of semi-glossy foliages to determine if conditioned by same genes. Continued development of new onion families segregating for health-enhancing fructans, male-fertility restoration, leaf waxiness, & bulb colors. Completed sequencing of onion complementary deoxyribonucleic acid (cDNAs) & presently identifying large numbers of single nucleotide polymorphisms in onion. Gynogenic haploids were previously extracted from hybrids from a cross of a doubled-haploid line with an inbred. These haploids were asexually propagated off of the basal plate & bulbils produced. These bulbils were planted in replicated trials & measured for health-enhancing fructans & flavonoids. DNA was isolated from over 200 haploids for genetic mapping of SNPs without heterozygosity. These markers will be used to more efficiently develop lines for commercial production. This will shorten time for hybrid development to reduce development costs & increase grower competitiveness. A series of crosses were completed to validate a major quantitative trait locus on chromosome 3 of cucumber that controls mitochondrial sorting. The source of this unique trait was sequenced & we are assembling the DNA sequence across the region on chromosome 3 to identify potential candidate gene(s) controlling mitochondrial sorting. A major grant from the USDA-SCRI program will support cloning the gene conditioning resistance to Zucchini Yellow Mosaic Virus in cucumber. Three cucumber mapping populations were developed for genetic mapping of fruit quality & yield-related genes. Two populations were observed & data collected for fruit quality & yield related traits including fruit number, size & flowering dates. Molecular markers were added for construction of a high-resolution genetic map. Machine trials were conducted in commercial fields & cucumber genomic resources are being developed which include whole genome sequencing & characterization. Resistance gene analogs in the cucumber genome were identified & mapped in the cucumber genome. Molecular mapping of genes for resistances against two cucumber pathogens are underway.
Kang, H., Yang, Y., Zhang, Z., Zhang, S., Mao, Z., Cheng, G., Gu, X., Huang, S., Weng, Y., Xie, B. 2010. Fine Genetic Mapping Localizes Cucumber Scab Resistance Gene Ccu into an R Gene Cluster. Journal of Theoretical and Applied Genetics. Available: http://www.springerlink.com/content/lqu520225154x357/.