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United States Department of Agriculture

Agricultural Research Service



2012 Annual Report

1a. Objectives (from AD-416):
Identify non-traditional plant resources and associated management methods with potential to enhance nutrition and health of small ruminants produced in central Appalachia. Specific objective 1: Identify plant resources and determine plant growth characteristics and management practices that can expand forage options. Specific objective 2: Identify plant resources and plant management strategies that can help control gastrointestinal helminths which infect small ruminants.

1b. Approach (from AD-416):
Controlled environment, field plot, and laboratory experiments will be conducted to determine growth habit, herbage yield, and chemical composition of non-traditional pasture legumes, forbs and grasses with potential for use as forage or forage supplements for small ruminants. Emphasis will be placed on plant species having physical properties or chemical constituents that disrupt the life cycle of the gastrointestinal nematode, Haemonchus contortus or provide immune system support for the ruminant. Non-traditional plant species used in these studies include condensed tannin-containing legumes, American potato bean, purslane, artemisia, and chicory. Condensed tannin-containing forage species will be evaluated for economic potential for hay production under central Appalachian growing conditions using criteria of yield, forage quality, and stand persistence. Native Appalachian plant species that have the potential to provide sufficient yield and bioactive constituents for small ruminants will be identified. The effect of plant species, management, and season on antioxidant capacity of traditional and non-traditional forage species will be measured using samples from multi-year field studies. The influence of edaphic and solar conditions on physical and chemical properties of plant resources will be determined using plants grown with and without mineral deficiencies, with varying levels of UV light, or with natural radiation attenuation. Potential adverse effects of non-traditional plant resources on rumen metabolism and potential alterations in bioactivity by rumen microbial activity will be assessed using in vitro rumen fermentation assays. Anthelmintic activity of plant materials and isolated constituents will be determined using in vitro and in vivo parasite assays. The effect of plant morphology, sward density, and canopy height on hatching and migration of parasite larvae will be evaluated to determine how physical characteristics of pastures can be manipulated to disrupt the parasite life cycle.

3. Progress Report:
Fiscal Year 2012 Consolidated Appropriations Act (H.R. 2112) passed by Congress and signed by the President on November 18, 2011, authorized the closing of the office/laboratory at Beaver, West Virginia. The program at Beaver was terminated retroactively to October 1, 2011. Therefore, this project is being terminated through the FY 2012 Annual Reports process.

4. Accomplishments

Review Publications
De Almeida, G.F., Horsted, K., Thamsborg, S.M., Kyvsgaard, N.C., Ferreira, J.F., Hermansen, J.E. 2012. Use of Artemisia annua as a natural coccidiostat in free-range broilers and its effects on infection dynamics and performance. Veterinary Parasitology. 186:178-187.

Katiki, L., Chagas, A., Takahira, R.K., Juliana, H.R., Ferreira, J.F., Amarante, A. 2012. Evaluation of Cymbopogon schoenanthus essential oil in lambs experimentally infected with Haemonchus contortus. Veterinary Parasitology. 186:312-318.

Ferreira, J.F., Zhelijazkov, V.D., Gonzalez, J.M. 2012. Artemisinin concentration and antioxidant capacity of Artemisia annua distillation byproduct. Industrial Crops and Products. 41:294-298.

Cassida, K.A., Foster, J.G., Gonzalez, J.M., Zobel, R.W., Sanderson, M.A. 2012. Available soil phosphorus affects herbage yield and stand persistence in forage chicory. Agronomy Journal. 104:807-816.

Squires, J.M., Ferreira, J.F., Lindsay, D.S., Zajac, A.M. 2010. Effects of artemisinin and Artemisia annua extracts on Haemonchus contortus in gerbils (Meriones unguiculatus). Veterinary Parasitology. 175:103-108.

Cassida, K.A., Lester, E.C., Foster, J.G., Turner, K.E. 2012. Recirculating elutriator for extracting gastrointestinal nematode larvae from pasture herbage samples. Veterinary Parasitology. 188(1-2):60-67.

Katiki, L.M., Chagas, A., Bizzo, H.R., Ferreira, J.F., Amarante, A. 2011. Anthelmintic activity of Cymbopogon martinii, Cymbopogon schoenanthus and Mentha piperita essential oils evaluated in four different in vitro tests. Veterinary Parasitology. 183:103-108.

Corbit, R., Ferreira, J.F., Ebbs, S., Murphy, L.L. 2005. Simplified cxtraction of ginsenosides from American ginseng (panax quinquefolius L.) for high-performance liquid chromatography-ultraviolet analysis. Journal of Agricultural and Food Chemistry. 53:9867-9873.

Tekippe, J.A., Hristov, A.N., Heyler, K.S., Cassidy, T.W., Zheljazkov, V.D., Ferreira, J.F., Karnati, S.K., Varga, G.A. 2011. Rumen fermentation and production effects of Origanum vulgare L. leaves in lactating dairy cows. Journal of Dairy Science. 94:5065-5079.

Brisibe, E.A., Uyoh, E.A., Brisibe, F., Magalhaes, P.M., Ferreira, J.F. 2008. Building a golden triangle for the production and use of artemisinin derivatives against falciparum malaria in Africa. African Journal of Biotechnology. 7(25):4884-4896.

Millar, K.D.L., Crandall-Stotler, B., Ferreira, J.F.S., Wood, K.V. 2007. Antimicrobial properties of three liverworts in axenic culture: Blasia pusilla, Pallavicinia lyellii and Radula obconica. Cryptogamie Bryologique. 28(3):197-210.

Ferreira, J.F., Laughlin, J.C., Delabays, N., Magalhaes, P.M. 2005. Cultivation and genetics of Artemisia annua L. for the increased production of the antimalarial artemisinin. Plant Genetic Resources. 3(2):206-229.

Marchese, J.A., Ferreira, J.F., Rehder, V., Rodrigues, O. 2010. Water deficit on the accumulation of biomass and artemisinin in annual wormwood (Artemisia annua L., Asteraceae). Brazilian Journal of Plant Physiology. 22(1):1-9.

Ferreira, J.F., Luthria, D.L., Sazaki, T., Heyerick, A. 2010. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with Artemisinin. Molecules. 15:3135-3170.

Last Modified: 2/23/2016
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