Location: Corn Host Plant Resistance Research2010 Annual Report
1a. Objectives (from AD-416)
Identify and develop corn germplasm with resistance to Aspergillus flavus infection/aflatoxin contamination and ear-feeding insects and release this germplasm together with information on molecular markers and methodology that will expedite its deployment into commercially available corn hybrids. Specific objectives include the following: (1) determine the effects of indigenous fungal species and ear-feeding insects on A. flavus infection and aflatoxin accumulation in corn grain; (2) identify new sources of corn germplasm with resistance to A. flavus infection and aflatoxin accumulation and/or resistance to damage by southwestern corn borer, fall armyworm, and corn earworm; (3) identify quantitative trait loci, genes, and proteins associated with resistance in corn to A. flavus infection, aflatoxin accumulation, and insect damage; and (4) enhance corn germplasm with resistance to A. flavus infection, aflatoxin accumulation, and insect damage and release germplasm lines as sources of resistance.
1b. Approach (from AD-416)
Objective 1. Determine the effects of indigenous fungal species and ear-feeding insects on A. flavus infection and aflatoxin accumulation in corn grain. Colonization of corn grain is rarely by a single fungal species, but rather a mixture of fungi. Fusarium verticillioides (syn. F. moniliforme) is the most commonly reported fungus infecting corn in the USA, and it is frequently found together with A. flavus. Acremonium zeae is a common contaminant of preharvest corn in the Southeast. It has been reported to suppress growth of both A. flavus and F. verticillioides in laboratory experiments. The interactions of these fungi will be investigated to determine whether F. verticillioides and A. zeae affect A. flavus infection of corn grain and the subsequent accumulation of aflatoxin, and if so, whether these fungi are impediments to the identification of aflatoxin-resistant corn germplasm. The association between insect damage and aflatoxin accumulation in different corn genotypes will be investigated and the extent to which resistance to damage by southwestern corn borer, Diatraea grandiosella; fall armyworm, Spodoptera frugiperda; or corn earworm, Helicoverpa zea, reduces aflatoxin contamination will be determined. Objective 2. Identify new sources of corn germplasm with resistance to A. flavus infection and aflatoxin accumulation and/or resistance to damage by southwestern corn borer, fall armyworm, and corn earworm. Corn germplasm from diverse backgrounds will be screened for resistance to A. flavus/aflatoxin, southwestern corn borer, fall armyworm, and corn earworm. Information on the effects of other fungi or insects on A. flavus/aflatoxin accumulation (Objective 1) will be used to refine and improve techniques for evaluating germplasm for resistance. Newly identified sources of resistance will be used to pursue Objectives 3 and 4. Objective 3. Identify quantitative trait loci, genes, and proteins associated with resistance in corn to A. flavus infection, aflatoxin accumulation, and insect damage. Populations of F2:3 families and recombinant inbred lines derived from crosses between aflatoxin or insect resistant inbred lines and susceptible lines will be used to identify quantitative trait loci (QTL) associated with resistance. Resistant and susceptible corn inbred lines and recombinant inbred lines will be used in complementary investigations to identify candidate genes and proteins associated with resistance. Molecular markers identified in these investigations will be used in developing improved germplasm lines (Objective 4). Objective 4. Enhance corn germplasm with resistance to A. flavus infection, aflatoxin accumulation, and insect damage and release germplasm lines as sources of resistance. Both breeding methods based on phenotypic performance and those based on molecular markers will be used to enhance germplasm with resistance to aflatoxin contamination and insect damage. The effectiveness of molecular markers based on QTL, genes, and proteins identified in Objective 3 in transferring resistance to A. flavus/aflatoxin and insect damage into germplasm lines with desirable agronomic qualities will be determined.
3. Progress Report
Germplasm accessions obtained through the Germplasm Enhancement of Maize (GEM) project and from the International Maize and Wheat Improvement Center (CIMMYT) were evaluated for resistance to aflatoxin accumulation in 2009. The lines exhibiting the lowest levels of aflatoxin were retained for inclusion in the breeding program. Hybrids were produced by crossing lines with different levels of resistance to Aspergillus (A.) flavus/aflatoxin to Bacillus thuringiensis (BT) and non-BT versions of proprietary lines. The hybrids were evaluated for aflatoxin accumulation and corn earworm damage in Mississippi, Georgia, and North Carolina. Damage from ear-feeding insects differed significantly among locations. Aflatoxin accumulation was generally lower in BT hybrids than in their non-BT versions; however, the interaction of inbred lines and BT/non-BT testers was not significant. A panel of 300 diverse lines from U.S. breeding programs and the CIMMYT was chosen for an association mapping project to identify genes associated with reduced aflatoxin accumulation. These lines were crossed with Va35, a susceptible line; the testcrosses were evaluated for A. flavus infection, aflatoxin accumulation, and corn earworm damage in field tests conducted in two locations in Mississippi and two locations in Texas (6406-21000-011-07S and 6406-21000-09S) in 2009 and again in 2010. The results should be useful in identifying genes and groups of genes associated with resistance to A. flavus/aflatoxin and in determining which lines possess unique genes for resistance. A diallel cross produced from eight parental inbred lines varying in level of resistance to aflatoxin accumulation was evaluated for Aspergillus ear rot, aflatoxin accumulation, and A. flavus biomass. Biomass was determined by quantitative real-time polymerase chain reaction (q-RT-PCR). A. flavus biomass and aflatoxin concentration were highly correlated (r = 0.90) in corn grain produced in the 2-year field trial. Estimates of general combining ability (GCA) effects for germplasm lines developed and released as sources of resistance to aflatoxin accumulation (Mp313E, Mp715, Mp717) indicated that these lines, when used in hybrids, contribute to both reduced aflatoxin and reduced fungal biomass. Experiments to identify quantitative trait loci (QTL) for resistance to A. flavus/aflatoxin in Mp313E, Mp715, and Mp717 provided valuable information on molecular markers for resistance to aflatoxin accumulation in corn. Additional genetic analyses and fine mapping of the QTL reduced their size and improved the identification of useful molecular markers for resistance to A. flavus/aflatoxin. The refined estimates of QTL associated with resistance are being used to develop near-isogenic lines from backcrosses of Mp313E, Mp715, and Mp717 to B73, T173, and Va35. Several lines selected from crosses between Mp313E × Va35 and Mp715 × Va35 exhibited not only high levels of resistance to aflatoxin accumulation, but also desirable agronomic qualities in several environments. Confirmation of QTLs associated with resistance to aflatoxin accumulation in the lines is underway.
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