Location: Wheat, Sorghum and Forage Research
Project Number: 5440-21220-027-00-D
Project Type: In-House Appropriated
Start Date: Feb 6, 2008
End Date: Feb 5, 2013
The long-term objectives of this project are the development of sorghum (Sorghum bicolor) germplasm lines with improved bioenergy, feed, and food value, and the elucidation of genetic, biochemical, and biological factors impacting these characters. Over the next five years, the following specific objectives will be addressed: 1) Identify and evaluate genes to improve sorghum for bioenergy, food, and feed traits, 2) Develop a better understanding of genes and fundamental mechanisms controlling cell wall formation and energy availability, and 3) Develop molecular and other technologies for monitoring sorghum fungal pathogens and determine the effects of sorghum genetic modification for bioenergy on pathogen populations.
The project utilizes a diverse set of technologies and approaches that are best delineated by objective: Objective 1 will be addressed primarily using traditional whole-plant plant breeding techniques, animal feeding trials, and established laboratory methods for assessment of feed and bioenergy value. Objective 2 will be addressed using current molecular and biochemistry technologies including PCR, RT-PCR, HPLC, microarrays, GC-MS. Objective 3 incorporates both field-based experiments and current molecular biology technologies. All experiments will utilize sound statistical designs to allow hypothesis testing at established levels of probability.