Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: STRAIN TYPING OF CHRONIC WASTING DISEASE (CWD) AND SCRAPIE BY INTRACEREBRAL INOCULATION INTO TRANSGENIC AND INBRED MOUSE LINES

Location: Animal Disease Research

Project Number: 5348-32000-030-01-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 28, 2007
End Date: Sep 27, 2012

Objective:
To identify and differentiate typical and atypical case samples of CWD and Scrapie by characterizing the biologic phenotype and pathologic profile of these agents when administered to susceptible lines of transgenic and inbred mice.

Approach:
Tissue samples from deer, elk, sheep, and goats with Transmissible Spongiform Encephalopathy (TSE) will be administered to mice by intracerebral injection. Multiple tissue types will be included, such as samples of brain, lymph node, blood, urine, feces, antler velvet, and muscle. Mouse models used as recipient hosts will include both preexisting and recently created transgenic and inbred mouse lines. Recipient mouse phenotype will be evaluated by measuring clinical response, population disease rate, incubation time, and pathologic profile within the central nervous system (CNS). Pathologic profile of CNS lesion foci is assessed by evaluating anatomic localization, spongiform change, astrocytic gliosis, and deposition of protease resistant prion protein. BSL-1; 9-4-09. Documents SCA with U. of WA.

Last Modified: 04/24/2017
Footer Content Back to Top of Page