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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Mycology and Nematology Genetic Diversity and Biology Laboratory » Research » Research Project #432592

Research Project: Improved Classification and Taxonomic Methods for Agriculturally Important Nematodes

Location: Mycology and Nematology Genetic Diversity and Biology Laboratory

Project Number: 8042-22000-299-00-D
Project Type: In-House Appropriated

Start Date: Mar 22, 2017
End Date: Nov 5, 2018

Objective 1: Assay and analyze the variability in DNA genetic markers across nematode taxa to ascertain those markers optimal for identifying priority crop-parasitic nematodes and for refining their phylogenies. Sub-objective 1.A: Molecular Genetic Markers applied to identify new nematode populations. Sub-objective 1.B: Mining BARC nematode biodiversity with molecular markers. Objective 2: Apply microscopic and DNA genetic marker methods to a diversity of crop parasitic nematode taxa and specimens to identify unknown specimens and to improve diagnostic approaches. Subobjective 2.A: Improved Microscopic Techniques. Subobjective 2.B: Updating and Collating Cyst Nematode and Dagger Nematode Taxonomy.

Expanding ribosomal and nuclear gene sequences to more species beyond the relative handful in GenBank, compared to the many known number of morphospecies, will produce more informative family trees that will demonstrate the distribution of plant parasitism for improved pathology prediction. Advanced microscopy techniques and appropriately selected and designed DNA genetic markers will reveal more detailed features and increase consistency of nematode description for improved diagnosis of described and new nematodes that may damage agriculturally important plants.