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Hidden insect infestations in wheat are very hard to detect and they can degrade the end-use quality and decrease the value of infested shipments. We have developed the software for the Perten SKCS 4100 that will allow it to detect wheat kernels that have hidden infestation. Live insect detection accuracy rates were 25% for kernels with small larvae, 62% for kernels with medium-sized larvae, 87% for kernels with large larvae, and 88% for kernels with pupae. The false positive error rate (selecting sound kernels as infected) was 0.5% or less depending on the wheat class.