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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food and Feed Safety Research » People & Locations » Leslie Scharfenstein (Les)

Leslie L Scharfenstein (Les)
Food and Feed Safety Research
Molecular Biologist

Phone: (504) 286-4374
Fax: (504) 286-4419
Room 2104

1100 ROBERT E. LEE BLVD.
BLDG 001 SRRC
NEW ORLEANS, LA 70124

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Prevalence of NRRL21882-like (Afla-Guard®) Aspergillus flavus on sesame seeds grown in research fields in the Mississippi Delta Reprint Icon - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Abbas, H.K., Bellaloui, N., Accinelli, C., Ebelhar, M.W. 2020. Prevalence of NRRL21882-like (Afla-Guard®) Aspergillus flavus on sesame seeds grown in research fields in the Mississippi Delta. Biocontrol Science and Technology. https://doi.org/10.1080/09583157.2020.1791798.
Genome sequence of an Aspergillus flavus CA14 strain that is widely used in gene function studies Reprint Icon - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Mack, B.M., Hua, S.T. 2019. Genome sequence of an Aspergillus flavus CA14 strain that is widely used in gene function studies. Microbiology Resource Announcements. 8(33):e00837-19. https://doi.org/10.1128/MRA.00837-19.
Identification of a copper-transporting ATPase involved in biosynthesis of A. flavus conidial pigment Reprint Icon - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Mack, B.M., Wei, Q., Gilbert, M.K., Lebar, M.D., Cary, J.W. 2019. Identification of a copper-transporting ATPase involved in biosynthesis of A. flavus conidial pigment. Applied Microbiology and Biotechnology. 103:4889-4897. https://doi.org/10.1007/s00253-019-09820-0.
The transcriptional regulator Hbx1 affects the expression of thousands of genes in the aflatoxin-producing fungus Aspergillus flavus Reprint Icon - (Peer Reviewed Journal)
Cary, J.W., Entwistle, S., Satterlee, T., Mack, B.M., Gilbert, M.K., Chang, P.-K., Scharfenstein, L.L., Yin, Y., Calvo, A. 2019. The transcriptional regulator Hbx1 affects the expression of thousands of genes in the aflatoxin-producing fungus Aspergillus flavus. G3, Genes/Genomes/Genetics. 9(1):167-178. https://doi.org/10.1534/g3.118.200870.
Aspergillus flavus GPI-anchored protein-encoding ecm33 has a role in growth, development, aflatoxin biosynthesis, and maize infection Reprint Icon - (Peer Reviewed Journal)
Chang, P.-K., Zhang, Q., Scharfenstein, L.L., Mack, B.M., Yoshimi, A., Miyazawa, K., Abe, K. 2018. Aspergillus flavus GPI-anchored protein-encoding ecm33 has a role in growth, development, aflatoxin biosynthesis, and maize infection. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-018-9012-7.
The Aspergillus flavus homeobox gene, hbx1, is required for development and aflatoxin production Reprint Icon - (Peer Reviewed Journal)
Cary, J.W., Harris-Coward, P.Y., Scharfenstein, L.L., Mack, B.M., Chang, P.-K., Wei, Q., Lebar, M.D., Carter-Wientjes, C.H., Majumdar, R., Mitra, C., Banerjee, S., Chanda, A. 2017. The Aspergillus flavus homeobox gene, hbx1, is required for development and aflatoxin production. Toxins. 9(10):315. https://doi.org/10.3390/toxins9100315.
Aspergillus flavus aswA, a gene homolog of Aspergillus nidulans oefC, regulates sclerotial development and biosynthesis of sclerotium-associated secondary metabolites - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Li, R.W., Arroyo-Manzanares, N., De Saeger, S., Diana Di Mavungu, J. 2017. Aspergillus flavus aswA, a gene homolog of Aspergillus nidulans oefC, regulates sclerotial development and biosynthesis of sclerotium-associated secondary metabolites. Fungal Genetics and Biology. 104:29-37.
Transcriptome of Aspergillus flavus aswA (AFLA_085170) deletion strain related to sclerotial development and production of secondary metabolites ()
Chang, P.-K., Scharfenstein, L.L., Mack, B.M., Li, R.W. 2017. Transcriptome of Aspergillus flavus aswA (AFLA_085170) deletion strain related to sclerotial development and production of secondary metabolites. National Center for Biotechnology Information (NCBI). Accession: SRP082149.
The Aspergillus flavus fluP-associated metabolite promotes sclerotial production - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Ehrlich, K., Diana Di Mavungu, J. 2016. The Aspergillus flavus fluP-associated metabolite promotes sclerotial production. Fungal Biology. 120:1258-1268.
The effect of 2-phenylethanol treatment on Aspergillus flavus transcriptome ()
Chang, P-K., Hua, S.T., Sarreal, S.L., Scharfenstein, L.L., Li, R.W. 2015. The effect of 2-phenylethanol treatment on Aspergillus flavus transcriptome. National Center for Biotechnology Information (NCBI). Accession: SRP056528.
High sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of Aspergillus oryzae Reprint Icon - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Solorzano, C.D., Abbas, H.K., Hua, S.-S. T., Jones, W.A., Zablotowicz, R.M. 2015. High sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of Aspergillus oryzae. International Journal of Food Microbiology. 200:66-71.
Aspergillus flavus Blast2GO gene ontology database: elevated growth temperature alters amino acid metabolism - (Peer Reviewed Journal)
Chang, P-K., Scharfenstein, L.L. 2014. Aspergillus flavus Blast2GO gene ontology database: elevated growth temperature alters amino acid metabolism. Journal of Genetics and Genome Research. 1:005.
Lack of aflatoxin production by Aspergillus flavus on a resistant peanut line is associated with delayed expression of aflatoxin genes - (Peer Reviewed Journal)
Zhang, H., Scharfenstein, L.L., Carter Wientjes, C.H., Chang, P., Zhang, D., Meng, X., Yu, J. 2015. Lack of aflatoxin production by Aspergillus flavus on a resistant peanut line is associated with delayed expression of aflatoxin genes. World Mycotoxin Journal. 8(3):335-340.
Peanut resistant gene expression in response to Aspergillus flavus infection during seed germination - (Peer Reviewed Journal)
Zhang, H., Scharfenstein, L.L., Zhang, D., Chang, P., Montalbano, B.G., Guo, B., Meng, X., Yu, J. 2014. Peanut resistant gene expression in response to Aspergillus flavus infection during seed germination. Journal of Phytopathology. 163(3):212-221.
Transcriptomic profiles of Aspergillus flavus CA42, a strain that produces small sclerotia, by decanal treatment and after recovery - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Mack, B.M., Yu, J., Ehrlich, K. 2014. Transcriptomic profiles of Aspergillus flavus CA42, a strain that produces small sclerotia, by decanal treatment and after recovery. Fungal Genetics and Biology. 68:39-47.
Transcriptomic profiling of decanal effects on Aspergillus flavus gene expression in development and secondary metabolism ()
Chang, P-K., Scharfenstein, L.L., Yu, J., Mack, B.M., Ehrlich, K.C., Losada, L., Nierman, W.C., Bhatnagar, D. 2014. Transcriptomic profiling of decanal effects on Aspergillus flavus gene expression in development and secondary metabolism. National Center for Biotechnology Information (NCBI). Accession: SRX471362.
Aspergillus flavus VelB acts distinctly from VeA in conidiation and may coordinate with FluG to modulate sclerotial production - (Peer Reviewed Journal)
Chang, P-K., Scharfenstein, L.L., Li, P., Ehrlich, K. 2013. Aspergillus flavus VelB acts distinctly from VeA in conidiation and may coordinate with FluG to modulate sclerotial production. Fungal Genetics and Biology. 58-59:71-79.
Deletion of the Aspergillus flavus orthologue of A. nidulans fluG reduces conidiation and promotes production of sclerotia but does not abolish aflatoxin biosynthesis - (Peer Reviewed Journal)
Chang, P-K., Scharfenstein, L.L., Mack, B.M., Ehrlich, K. 2012. Deletion of the Aspergillus flavus orthologue of A. nidulans fluG reduces conidiation and promotes production of sclerotia but does not abolish aflatoxin biosynthesis. Applied and Environmental Microbiology. 78(21):7557-7563.
Identification of genetic defects in the atoxigenic biocontrol strain Aspergillus flavus K49 reveals the presence of a competitive recombinant group in field populations Reprint Icon - (Peer Reviewed Journal)
Chang, P.-K., Abbas, H.K., Weaver, M.A., Ehrlich, K., Scharfenstein, L.L., Cotty, P.J. 2012. Identification of genetic defects in the atoxigenic biocontrol strain Aspergillus flavus K49 reveals the presence of a competitive recombinant group in field populations. International Journal of Food Microbiology. 154:192-196.
Effects of laeA deletion on Aspergillus flavus conidial development and hydrophobicity may contribute to loss of aflatoxin production - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Ehrlich, K., Wei, Q., Bhatnagar, D., Ingber, B.F. 2012. Effects of laeA deletion on Aspergillus flavus conidial development and hydrophobicity may contribute to loss of aflatoxin production. Fungal Biology. 116:298-307.
Loss of msnA, a putative stress regulatory gene, in Aspergillus parasiticus and Aspergillus flavus increased production of conidia, aflatoxins and kojic acid - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Luo, M., Mahoney, N.E., Molyneux, R.J., Yu, J., Brown, R.L., Campbell, B.C. 2011. Loss of msnA, a putative stress regulatory gene, in Aspergillus parasiticus and Aspergillus flavus increased production of conidia, aflatoxins and kojic acid. Toxins. 3:82-104.
HypC, the anthrone oxidase involved in aflatoxin biosynthesis - (Peer Reviewed Journal)
Ehrlich, K., Li, P., Scharfenstein, L.L., Chang, P.-K. 2010. HypC, the anthrone oxidase involved in aflatoxin biosynthesis. Applied and Environmental Microbiology. 76(10):3374-3377.
Development and refinement of a high-efficiency gene-targeting system for Aspergillus flavus - (Peer Reviewed Journal)
Chang, P.-K., Scharfenstein, L.L., Wei, Q., Bhatnagar, D. 2010. Development and refinement of a high-efficiency gene-targeting system for Aspergillus flavus. Journal of Microbiological Methods. 81:240-246.
Absence of the Aflatoxin Biosynthesis Gene, norA, allows accumulation of deoxyaflatoxin B1 in Aspergillus flavus cultures - (Peer Reviewed Journal)
Ehrlich, K., Chang, P., Scharfenstein, L.L., Cary, J.W., Crawford, J.M., Townsend, C.A. 2010. Absence of the Aflatoxin Biosynthesis Gene, norA, allows accumulation of deoxyaflatoxin B1 in Aspergillus flavus cultures. FEMS Microbiology Letters. 305:65-70.
Are the Genes nadA and norB Involved in Formation of Aflatoxin G1 - (Peer Reviewed Journal)
Ehrlich, K., Scharfenstein, L.L., Montalbano, B.G., Chang, P. 2008. Are the Genes nadA and norB Involved in Formation of Aflatoxin G1. International Journal of Molecular Sciences. 9:1717-1729.